Source: Annual Congress 2006 - Pneumonia and other invasive pulmonary infections in children
Disease area: Paediatric lung diseases, Respiratory infections

Abstract

RRTI are very frequent in young children. RRTI can be a consequence of altered function of immune cells including neutrophils. The goal of the study was to evaluate the process of neutrophil activation via Ca mediated transduction signal into those cells. The studies was performed with the use of peripheral blood neutrophils from 41 RRTI children (21 girls, 20 boys) 3 to 10 years old with more than five episodes of RRTI per year and from control group of 30 healthy children age and sex matched free from allergic, immune and hematological disorders. Neutrophils were activated by bacterial peptide fMLP, opsonised zymosan (OZ) and phorbol myristat acetate (PMA). Intracellular Ca concentration kinetics was assessed by flow cytometry (Coulter Epics XL) with the use of Fluo3 and Fura Red fluorescent dyes. fMLP and OZ induced Ca mobilization lasted shorter in RRTI group (p<0.05).The peak influx of free Ca and its concentration in resting state after stimulation with fMLP were lower in patients (p<0.05). In RRTI group stimulation with OZ was delayed comparing to control (p<0.01). In response to PMA free Ca concentration decreased faster. The kinetic slopes of Ca concentration in both examined groups differed statistically in all measured points. Decrease on intracellular Ca concentration after PMA stimulation was higher (p<0.01) and lasted longer in RRTI group. Increased sensitivity to infections in RRTI children may be related to the disturbances in neutrophil activation via intracellular Ca concentration and subsequent production of free oxygen radicals. Those disturbances can be inherited or secondary to the infection or antibiotics therapy.

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Citations should be made in the following way:
B. Jakubczak, U. Demkow, K. Popko, M. Wasik (Warsaw, Poland). Disturbances in calcium (Ca) mediated signal transduction into neutrophils of children with recurrent infections of respiratory tract (RRTI). Eur Respir J 2006; 28: Suppl. 50, 1788

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