Source: Annual Congress 2006 - Pneumonia and other invasive pulmonary infections in children
Disease area: Paediatric lung diseases, Respiratory infections

Abstract

Introduction: We recently described the utility of selected ion flow tube mass spectrometry (SIFT-MS) as a diagnostic tool for detection of Pseudomonas aeruginosa (PA) in vitro (1). We undertook this study to determine whether breath samples from children with cystic fibrosis colonised with PA had characteristic patterns of volatile compounds which were detectable by SIFT-MS.
Methods: Children aged 6-18 years were eligible for participation. Breath was collected into single-use, non-adsorbent bags and transferred immediately to the laboratory for analysis using SIFT-MS. Breath samples were taken from children with cystic fibrosis (n=17) and asthma (n=6).
Results: Using SIFT-MS a large number of volatile compounds are detectable and accurately quantifiable in human breath. These include acetone, acetaldehyde, ethanol, ammonia and methanol. Children with CF had significant elevations of 2-propanol compared to those with asthma (mean 1940 ppb 95% CI 300-3570 vs mean 90 ppb 95% CI 2-180 p<0.01) and marginal elevations in hydrogen cyanide (mean 13 ppb 95% CI 8-19 vs mean 4 ppb 95% CI 0-10 p<0.05).
Conclusions: Breath analysis is feasible and safe in children with asthma and cystic fibrosis. 2-propanol and hydrogen cyanide show promise as biomarkers of bacterial infection in vivo.
1. Carroll WD et al. Detection of volatile compounds emitted by P aeruginosa using SIFT-MS. Pediatr Pulmonol 2005;16:32-9.

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Citations should be made in the following way:
W. D. Carroll, T. S. Wang, W. Lenney, P. Spanel, P. Boit, D. Smith (Stoke-on-Trent, Keele, United Kingdom; Prague, Czech Republic). Detection of elevated 2-propanol in breath samples from children with cystic fibrosis. Eur Respir J 2006; 28: Suppl. 50, 1780

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