Source: Annual Congress 2008 - Diagnosis of tuberculosis: problems and perspectives
Disease area: Respiratory infections

Abstract

INTRODUCTION
Diagnostic of mycobacteriosis caused by nontuberculous mycobacteria (NTM) is very difficult,because its symptoms in major cases are similar with tuberculosis.Main criteria in diagnostic of this condition is to isolate culture of NTM and their species identification.But full time of identification process is very long and it takes about 6 months.Indisputably,the using of molecular technique permits to reduce this period to 24 hours.
MATERIALS AND METHODS
A total of 119 strains (28 reference strains and 91 clinical isolates) submitted for identification from January to November 2007 were tested using culture and biochemical characteristics and hsp65-based RFLP identification.Specimens were submitted on solid medium, from which a DNA could directly be prepared.Like control method in this study we was used sequencing of the PCR product.
RESULTS
In our work we received 100% coincidence with results of identification of reference strains using three methods (biochemical examination,RFLP,sequencing). As regards for species identification of clinical isolates our results are as following: M.tuberculosis complex 25,3%,M.fortuitum/M.chelonae 23,1%,M.avium 15,4%,M.kansasii 12%,M.xenopi 9,9%,M. intracellulare 8,8%,M.gordonae 1,1%,M.scrofulaceum 1,1%,unknown species 2,2%,negative result 1,1%.In one case there was received negative result and it was established that this culture relates to Nocardia.2 NTM cultures we couldn‘t identify using biochemical testes and RFLP,because obtained biochemical characteristics and restriction patterns didn‘t conform for any reference strains,which were available in our laboratory.Species identification of clinical isolates NTM,showed the same data distribution as in other countries of the world.

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Citations should be made in the following way:
M. Krasnova, M. Makarova (Moscow, Russian Federation). Species identification of mycobacteria in Moscow state. Eur Respir J 2008; 32: Suppl. 52, 2393

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