Opportunities of bacteriological diagnostics of a tuberculosis of a lung in a modern stage

N. N. Parpieva, R. S. Muhamedov, K. S. Muhamedov, I. K. Muhamediyev (Tashkent, Uzbekistan)

Source: Annual Congress 2008 - Diagnosis of tuberculosis: problems and perspectives
Session: Diagnosis of tuberculosis: problems and perspectives
Session type: Thematic Poster Session
Number: 2382
Disease area: Respiratory infections

Congress or journal article abstract

Abstract

The Purpose: study of the efficiency of the use the different methods of the research for determination the diagnosis at the patients with lung tuberculosis.
The Material and methods of the study: examination of the sputum of the 50 patient with different forms of the lung tuberculosis. The Methods of the examination: bacterioscopy, sputum inoculation and method Polymerases chain reaction (PCR).
It is explored 20 patients revealed first time and 30 patients with the tuberculosis of the lung earlier treated. The patients were divided into 2 groups: first group: 24 patients with positive result of the sputum for MBT finding by method bacterioscopy. Second group: 26 patients with negative result of the sputum for MBT by method bacterioscopy. It is received following results by the method of the sputum inoculation: culture growth has been found only at 27 patients, in the first group at 24 patients, in 2 groups at 3 patients. The Analysis of PCR has shown the positive result in 1 group at 24 (100%), and in 2 groups amongst patients with negative analysis by bacterioscopy of the sputum, at 8 patients (30,8%) by method of PCR in the analysis of the sputum are discovered MBT, that is connected with poor allocation of bacteria. The Absence of the growing colony by method of the sputum inoculation possible in connection with: loss MBT abilities to growing in vitro;
Thereby, PCR-diagnostics tuberculosis allows diagnosing the active tuberculosis of the lung in several times quicker and effecter, than bacteriological method, determination MBT positive sick with poor allocation of bacteria.


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N. N. Parpieva, R. S. Muhamedov, K. S. Muhamedov, I. K. Muhamediyev (Tashkent, Uzbekistan). Opportunities of bacteriological diagnostics of a tuberculosis of a lung in a modern stage. Eur Respir J 2008; 32: Suppl. 52, 2382

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