Source: Annual Congress 2008 - Mechanisms of respiratory infections: interaction between the pathogen and the host
Disease area: Respiratory infections

Abstract

Invasive candidosis is a feared complication after lung transplantation. The incidence of Non-Candida albicans infections has increased due to the extended use of fluconazole. After being mismatched for years with C. (Candida) albicans, in 1995 C. dubliniensis was described as new species. C. dubliniensis is associated with invasive disease in immunocompromised hosts.
The unambiguous identification of this organism is a problem. Many of the fast and cheap methods for isolation, characterization and discrimination of Candida species lack sensitivity and specificity. Molecular genetic assays demonstrate a higher sensitivity and specifity but are more labor intensive and expensive.
The aim of our work was to compare several established methods for the identification of C. dubliniensis.
We used a panel of different methods for the characterization of 199 clinical isolates obtained from 122 clinical oropharyngeal samples. The results obtained with cultivation methods were compared with the AP-PCR which was considered the gold standard for these experiments. We achieved a sensitivity of 14 % for the identification on CHROMagar®, 42 % for the identification on rice agar, 100 % for discrimination on bird-seed agar, 100 % with an assimilation profile index (API ID 32C) and 97 % when grown at 45°C.
From our experiments we conclude that a panel of different identification methods is necessary to achieve a high level of sensitivity and specficity for the identification of C. dubliniensis. According to these results we propose an optimized flow chart for a clear-cut discrimination of C. dubliniensis from C. albicans.

Rating:

Share or cite this content

Citations should be made in the following way:
J. Pasligh, C. Radecke, M. Fleischhacker, C. Witt, M. Ruhnke (Berlin, Germany). Comparison of methods for the isolation and identification of candida dubliniensis. Eur Respir J 2008; 32: Suppl. 52, 2293

Member's Comments

No comment yet.

Related content which might interest you: Characterising pseudomonas carriage patterns using molecular typing. Source: International Congress 2019 – Infection and inflammation in cystic fibrosis Year: 2019 Novel approach to species identification of nontuberculous mycobacteria Source: Annual Congress 2010 - Nontuberculous mycobacterial infections and tuberculosis Year: 2010 Effects of PC945, a novel antifungal agent optimised for lung delivery, on Candida albicans lung infection in mice Source: Virtual Congress 2020 – Translational science in respiratory infections Year: 2020 Frequency of candida species mycoses isolation from patients with pulmonary tuberculosis Source: Eur Respir J 2004; 24: Suppl. 48, 422s Year: 2004 Influence of candida species fungi on growth and pathogenicity of mycobacteria tuberculosis Source: Eur Respir J 2007; 30: Suppl. 51, 663s Year: 2007 Non-invasive detection of Pseudomonas aeruginosa Source: Eur Respir J 2005; 26: Suppl. 49, 640s Year: 2005 Comparison of performance in two diagnostic methods for M. tuberculosis infection Source: Annual Congress 2008 - New tools in tuberculosis diagnosis Year: 2008 Whole Exome Sequencing study in a subgroup of sarcoidosis patients presenting an opportunistic infection with Cryptococcus neoformans. Source: Virtual Congress 2020 – Genetics and biomarkers for diagnosis and prognosis of granulomatous lung diseases Year: 2020 Applying clinical metagenomics for the detection and characterisation of respiratory infections Source: Eur Respir Monogr 2019; 83: 35-49 Year: 2019 Comparative study of S. milleri as a cause of community acquired pneumonia Source: Eur Respir J 2006; 28: Suppl. 50, 373s Year: 2006 HIRA-TAN: a real-time PCR-based diagnostic test for the pathogens of pneumonia Source: Annual Congress 2009 - Aetiology and treatment of community-acquired pneumonia Year: 2009 Is early serological investigation an useful diagnostic tool in pneumonia by M. pneumoniae? Source: Eur Respir J 2005; 26: Suppl. 49, 410s Year: 2005 Antibiotic susceptibility testing on streptococcus pneumoniae strains obtained from respiratory tract specimens Source: Eur Respir J 2001; 18: Suppl. 33, 139s Year: 2001 General evaluation in cases with community acquired pneumonia due to C. pneumoniae Source: Eur Respir J 2007; 30: Suppl. 51, 677s Year: 2007 Comparison of rapid in-house methods for drug susceptibility testing of mycobacterium tuberculosis with gold standards Source: Annual Congress 2007 - Research perspectives: diagnosis and treatment of tuberculosis Year: 2007 Identification of pleural infection microbiological patterns by applying next generation sequencing and bioinformatics analysis Source: Virtual Congress 2020 – Antibiotic therapy for pneumonia and pleural infections Year: 2020 Usefulness of ELISA in the multiple serodiagnosis of environmental mycobacteria infections Source: Annual Congress 2008 - Aetiology of respiratory infections: mycobacteria and fungi Year: 2008 Effect of Cryptococcus neoformans on the immune system of immunocompetent patients Source: Annual Congress 2012 - Prognostic indices in respiratory infections Year: 2012