Source: Annual Congress 2008 - Mechanisms of respiratory infections: interaction between the pathogen and the host
Disease area: Respiratory infections

Abstract

Background: CAP from Streptococcus pneumoniae is a pubic-health problem. PspA is the most promising protein candidate for the developing cost-effective protein-based vaccines against S.pneumoniae.
Objective: In this study, as parts of an ongoing pneumococcal surveillance study in China, analysis of genetic diversity is the necessary step in determining the components needed in the PspA vaccine which could elicit effective cross-protective coverage in China.
Methods: PspA genes of 82 S.pneumoniae isolates from health people (n = 38) and CAP patients (n= 44) were analyzed by the polymerase chain reaction and the PspA serologic dot-blot techniques.
Results: Based on the sequence differences, the classification of these PspA genes were subdivided into three families by comparison of sequences with reference strains according to Hobbllingshead et al.: 95.12% (n =78) of S. pneumoniae isolates could be assigned to family1 (n=40)and family2 (n=38). A dendrogram comparing the genetic relationship between the prevalence of PspA families in healthy and CAP people showed no statistically significant difference (P>0.05). All lysates of strains could cross- react with the same family antiserum, and little partly cross-react with others families.
Conclusion: Our results found that the PspA remain family types from S.pneumoniae isolates from CAP patients in China are in accordance to the limited information on PspA family distribution from other countries, the antiserum cross-reacting in the same family supports the hypothesis that the human PspA vaccine covering both PspA families 1 and 2 could be broadly cross-effective against S. pneumoniae.

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Citations should be made in the following way:
Q. Zhang, L. Qian, X. Zeng, X. Xie, C. Wang (Chongqing, China). Genetic diversity of pneumococcal surface protein A (PspA) in Streptococcus pneumoniae isolates from community acquired pneumonia (CAP) in china. Eur Respir J 2008; 32: Suppl. 52, 2299

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