Source: Annual Congress 2008 - Mechanisms of respiratory infections: interaction between the pathogen and the host
Disease area: Airway diseases, Respiratory infections

Abstract

Introduction: A. fumigatus is a frequent colonizer in patients with asthma and chronic lung diseases (e.g. cystic fibrosis). The aim of this study was to investigate the ability of A. fumigatus to form a biofilm-like matrix in vitro on human bronchial epithelia cells.
Methods: A. fumigatus ATCC #9197 was incubated on 16HBE and CFBE41o- in MEM at different pH, concentrations of FBS, temperature, production time and different flow conditions. Dry weight measurement and antifungal drug susceptibility testing was performed. Scanning electron microscopy (SEM) and confocal scanning laser microscopy (CSLM) images were analyzed.
Result: The dry weight of the produced biofilm exceeded 7.4 mg on 16HBE and 7.7 mg on CFBE41o- cells after 72h of biofilm production. There was no significant difference in dry weight increase between the cell lines. All antifungal drugs were weakened or displayed resistance (>8µg/ml) against Aspergillus embedded in biofilm. The SEM pictures displayed a network of hyphal structures and matrix at 48h. Characteristic flow channels were observed at 72h. CSLM images displayed attached conidia on the cells after 4h, conidia and hyphal structures after 24h and matrix formations after 72h. A-Alexafluor dyed polysaccharides of the cell wall and of the ECM in the biofilm. Three dimensional constructs of the CSLM pictures displayed biofilm on 16HBE and proofed viability of the cells after 48h co-incubation. Differences in biofilm production between 16HBE and CFBE41o- were not significant.
Conclusions: A. fumigatus is able to form a biofilm structure in vitro on bronchial epithelia cells. Potential clinical implications of A. fumigatus biofilm formation in vivo require further investigations.

Rating:

Share or cite this content

Citations should be made in the following way:
M. Seidler, S. Salvenmoser, F. M. Mueller (Heidelberg, Germany). Biofilm production of Aspergillus fumigatus on bronchial epithelia cells. Eur Respir J 2008; 32: Suppl. 52, 2288

Member's Comments

No comment yet.

Related content which might interest you: Aspergillus fumigatus conidia induce interferon-ß signalling in respiratory epithelial cells Source: Eur Respir J 2012; 39: 411-418 Year: 2012 Aspergillus fumigatus conidia induce internalization dependent IFN-beta and IP-10 expression in airway epithelial cells Source: Eur Respir J 2005; 26: Suppl. 49, 554s Year: 2005 Interleukin-10 genotypes and airways colonization by Aspergillus fumigatus in cystic fibrosis patients Source: Eur Respir J 2005; 26: Suppl. 49, 403s Year: 2005 IL-10 induced T-cell tolerance to Aspergillus fumigatus and Pseudomonas aeruginosa in cystic fibrosis Source: Eur Respir J 2001; 18: Suppl. 33, 8s Year: 2001 Evidence for local IgE production to Aspergillus fumigatus in children with cystic fibrosis Source: Annual Congress 2009 - Cystic fibrosis: understanding a complex disease Year: 2009 Parasexual recombination enables Aspergillus fumigatus to persist in cystic fibrosis Source: ERJ Open Res, 6 (4) 00020-2020; 10.1183/23120541.00020-2020 Year: 2020 Biofilm production and adherence to airway epithelial cells of P. aeruginosa strains: effect of N-acetyl-cysteine Source: Eur Respir J 2005; 26: Suppl. 49, 726s Year: 2005 Coinfection with Pseudomonas aeruginosa and Aspergillus fumigatus in cystic fibrosis Source: Eur Respir Rev, 29 (158) 200011; 10.1183/16000617.0011-2020 Year: 2020 Impact of Aspergillus fumigatus infection on lung function in children with cystic fibrosis Source: Virtual Congress 2021 – Cystic fibrosis in children: various aspects Year: 2021 Aspergillus fumigatus compromise the respiratory epithelium barrier by disruption of tight junctions and activation of PAR-2 Source: Annual Congress 2008 - Novel therapeutic approaches and immunological mechanisms in airway cell pathobiology Year: 2008 Transmission-frequency of Aspergillus fumigatus in cystic fibrosis patients through coughing Source: International Congress 2018 – Cystic fibrosis in adults: current research Year: 2018 Volatile organic compounds emitted by pseudomonas aeruginosa and aspergillus fumigatus mono-cultures and in co-culture Source: International Congress 2015 – Cystic fibrosis: physiology, biomarkers, microbiology and miscellaneous Year: 2015 Tracheal oxalosis associated with Aspergillus niger tracheobronchitis Source: Eur Respir J 2013; 41: 995-997 Year: 2013 In vivo evidence for aspergillus fumigatus and pseudomonas aeruginosa driven th-17 type inflammatory responses in the lungs of CF-patients Source: Annual Congress 2009 - Cystic fibrosis: understanding a complex disease Year: 2009 Sensitisation to Aspergillus fumigatus as a risk factor for bronchiectasis in COPD Source: International Congress 2017 – Towards personalised medicine in COPD: biological and clinical phenotypes Year: 2017 Aspergillus and other fungi Source: ERS Course 2016 Year: 2016 Aspergillus and other fungi Source: School Course 2012 - Lung Transplantation Year: 2012 Clinical application and radiological relavance for Aspergillus fumigatus- and flavus-specific IgG in chronic Pulmonary Aspergillus infection> Source: Virtual Congress 2020 – Aspergillosis and chronic lung infections Year: 2020