Source: Annual Congress 2008 - Mechanisms of respiratory infections: interaction between the pathogen and the host
Disease area: Airway diseases, Respiratory infections

Abstract

SIGIRR, a member of Toll-like receptor(TLR)–interleukin 1 receptor superfamily that highly expressed in the airway epithelial cells.when the pathogenic microorganism invades the airway, it negatively modulates native immune responses especially in the earlier period of the inflammatory reaction.
Methods
A mouse model of the acute airway inflammation was established by flagellin inhalation. Then the mice were infected with lentivirus packaged recombination PLL-SIGIRR through inhalation way, The mRNA and protein expression level of SIGIRR and TLR5 in each group were detected by RT-PCR and Western Blotting. The activity of NF-κB was determined by EMSA, and the concentration of IL-6 and TNF-α in the mice peripheral blood were assayed by ELISA.
Results
The injury of lung in the control group was more serious than in the experimental group. SIGIRR expression in the airway epithelial cells during experimental group increased compared to the control group, but TLR5 expression reduced, and both had the decline trend along with the time extension. Moreover, the expression level of cytokine IL-6 and TNF-α in experimental group is more remarkable reduction than the control group, and the activity of NF-κB decreased significantly (P<0.05).
Conclusion
Overexpression of SIGIRR in the airway has the protective function to the airway inflammation caused by flagellin, particularly overexpression in advance may has more obvious protective effect compared to post infection.The negative correlation between SIGIRR and TLR5 expression indicated that its protective function was possibly realized in the manner of the adjustment of TLR5 expression.

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Citations should be made in the following way:
X. Wu, Y. Zhao, C. Zhang, G. Qian (Chongqing, Nanjing, Jiangsu Province, China). The protective effects of SIGIRR on flagellin-induced acute inflammation of the airway in mice. Eur Respir J 2008; 32: Suppl. 52, 2287

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