Source: Annual Congress 2008 - Mechanisms of respiratory infections: interaction between the pathogen and the host
Disease area: Respiratory infections

Abstract

The bark of aspen is a waste in the paper pulp industries, that can be used as cheap and perspective raw material for extraction of biological active compounds.
The aim of the experiments was the comparative study of antibacterial action of Populus tremula L. bark‘s extracts.
Methods. Lipids have been extracted from the origin samples using petroleum ether (lipids I) and diethyl ether (lipids II) in Soxletus apparatus. The isopropanol extracts were prepared at room temperature. Two methods were used for definition of bacteria‘s sensitivity: a) method of diffusion in agar with application of the disks impregnated with the tested samples (for 1) Bacillus subtilus; 2) Escherichia coli) and b) method of serial cultivations (for 3) Streptococcus pneumoniae and 4) Haemophilus influenzae).
Results. The highest activity among the investigated samples against bacteria 1,2 possesses lipids II from aspen‘s rhytidome (1 – 10 mg/ml). Lipids I of aspen‘s bark do not show activity against bacteria 1,2. Isopropanol extracts of aspen bark suppress the growth of bacteria 1 – 4 at high concentrations only (20 – 30 mg/ml). Lipids I and lipids II showed high antibacterial activity against S. pneumoniae. The minimum inhibiting concentrations (MIC) for S. pneumoniae lipids I was 0,5 – 50 mg/ml and lipids II 0,0005 – 5,0 mg/ml. Antibacterial activity of rhytidome extracts was a little bit higher, than phloem extracts. MIC for H. influenzae lipids was 30 – 50 mg/ml. Long storage strains in a refrigerator reduces antibacterial activity of the investigated extracts and MIC grows up to 50 mg/ml.
Conclusion. It is perspective to use lipids of aspen bark as a part of development of medicines for pulmonary patients with acute diseases.

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Citations should be made in the following way:
N. Faustova, M. Faustova, I. Deineko (Saint-Petersburg, Russian Federation). In vitro antibacterial activity bark‘s extracts of populus tremula L. Eur Respir J 2008; 32: Suppl. 52, 2294

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