Source: Annual Congress 2006 - Virus-induced respiratory tract infection
Disease area: Airway diseases, Respiratory infections

Abstract

Aim To investigate the characteristics, distribution and response to vaccination of CD4⁺ T cells to respiratory pathogens such as influenza (FLU), respiratory syncytial virus (RSV) and non-typeable Haemophilus influenzae (NTHi) in patients with COPD and healthy subjects. Methods Determination of the precursor frequencies for FLU and RSV, and NTHi outer membrane protein P6 by antigen-induced in vitro expansion of total PBMC in healthy individuals (n=9) and patients with COPD (n=16). Analysis of the CD27 and CCR7 expression of antigen-specific CD4⁺ T cells and their ability to produce IFNgamma and IL-2 after direct ex-vivo stimulation. Analysis of the distribution of FLU-specific CD4⁺ T cells between the blood and lung tissue T-cell pool. Results We found that FLU-, RSV-, and NTHi specific CD4⁺ memory T cells occurred in low frequencies in the peripheral blood of healthy individuals and patients. RSV- and NTHi-specific CD4⁺ T cells had a central memory phenotype as characterised by high CD27 and CCR7 expression and had the ability to expand upon antigen-specific restimulation in vitro. We did, however, not observe an expansion of the CD4⁺ memory T cell pool upon influenza-vaccination. FLU-specific CD4⁺ T cells occurred in higher frequency in the lung tissue where they exhibited a differentiated phenotype with a downmodulation of CD27. Conclusion No gross defects were found in circulating CD4⁺ memory cells specific for pathogens associated with COPD. However, since a large fraction of differentiated memory cells resided in the lung, future studies should aim to clarify their function in protective immunity. Supported by the Dutch Asthma Foundation, the Dutch Foundation for Asthma Prevention, and Solvay Farmaceuticals.

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G. J. de Bree, H. Daniëls, M. van Schilfgaarde, H. M. Jansen, T. A. Out, R. A. W. van Lier, R. E. Jonkers (Amsterdam, Alkmaar, Bilthoven, Netherlands). CD4+ memory T cells specific for common respiratory pathogens. Eur Respir J 2006; 28: Suppl. 50, 968

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