Source: Annual Congress 2006 - Clinical aspects of tuberculosis

Abstract

The aim of the study was investigation of nitric oxide (NO) generation changes in blood mononuclear cells (M) and neutrophils (N) of patients with different course (new revealed and relapse) of pulmonary tuberculosis.
Materials and methods: we investigated leukocytes of 73 patients with new revealed pulmonary tuberculosis (group 1) and 92 patients with relapse of pulmonary tuberculosis (group 2). The control group consisted of 20 healthy volunteers. Concentration of NO stable final metabolites – nitrite in M and N were determinated by spectrophotometric method using Greiss reaction. Measurements were performed in native cells and after 20 hours of incubation with killed BCG culture and were followed by calculation of stimulation coefficient (SC).
Results: It was established that basal and stimulate level of NO was significantly higher in M and N of patients group 1 in comparison with control group. SC was normal. Basal level of NO in M of patients group 2 was also significantly higher than in control. Response to stimulation in M was normal and accordingly SC was reduced. In contrast basal level of NO in N was reduced and SC was higher.
Conclusion: Change in level NO generation in blood leukocytes is the first defensive reaction against infection and obeys to the different laws in patients with different course of pulmonary tuberculosis.

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Citations should be made in the following way:
R. Y. Abdullayev, O. G. Komissarova, G. O. Kaminskaya (Moscow, Russian Federation). Level of nitric oxide metabolites in blood mononuclear cells and neutrophils of patients with pulmonary tuberculosis. Eur Respir J 2006; 28: Suppl. 50, 832

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