Late Breaking Abstract - Intra-alveolar neutrophil-derived microvesicles: a biomarker of COPD severity
J. Garner (London, United Kingdom), S. Soni (London, United Kingdom), K. O'Dea (London, United Kingdom), K. Srikanthan (London, United Kingdom), E. Tenda (London, United Kingdom), A. Aboelhassan (London, United Kingdom), S. Singh (London, United Kingdom), S. Kemp (London, United Kingdom), M. Wilson (London, United Kingdom), O. Usmani (London, United Kingdom), P. Shah (London, United Kingdom), M. Takata (London, United Kingdom)
Source: International Congress 2018 – Advanced profiling of immune cells in chronic lung diseases
Disease area: Airway diseases
Abstract
Background: Microvesicles (MV) play an important role in mediating intra-alveolar inflammation during acute lung injury (Soni et al. Thorax 2016). We investigated the profile of MVs present within bronchoalveolar lavage fluid (BALF) and plasma in patients with another inflammatory disease, COPD, and assessed relationship to disease severity.
Methods: Moderate to severe COPD patients (n=30) underwent clinical evaluation (symptom scores, lung function, exercise testing) and bronchoscopy. Paired plasma/BALF samples were analysed by flow cytometry for MVs derived from leucocytes (CD45+), neutrophils (CD66b+/CD11b+), monocytes (CD45+/CD14+), alveolar macrophages (CD206+/CD71+), platelets (CD31+/42b+), epithelial (EpCAM+/T1a+) and endothelial cells (CD146+/CD62E+/CD144+).
Results: We identified MV subpopulations within BALF (leukocyte, neutrophil, monocyte, alveolar macrophage, epithelial, platelet) and plasma (leukocyte, neutrophil, monocyte, endothelial) samples. Only BALF neutrophil MV numbers correlated with modified MRC dyspnoea scale (p<0.001); St George’s Respiratory questionnaire (p<0.05); FEV1 (p<0.05); 6 min walk test (p<0.01); and BODE mortality index (p<0.001). Neutrophil numbers in BALF/plasma did not correlate with any indices.
Conclusions: We have undertaken for the first time a comprehensive evaluation of MVs within BALF/plasma of COPD patients, and demonstrated BALF neutrophil-derived MV numbers correlate with functional and clinically relevant disease severity indices. Our data strongly suggest BALF neutrophil MVs are a novel, clinically relevant biomarker for COPD disease severity, and may have a pathogenic role in COPD progression and be a potential target to modulate the inflammation in COPD.
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J. Garner (London, United Kingdom), S. Soni (London, United Kingdom), K. O'Dea (London, United Kingdom), K. Srikanthan (London, United Kingdom), E. Tenda (London, United Kingdom), A. Aboelhassan (London, United Kingdom), S. Singh (London, United Kingdom), S. Kemp (London, United Kingdom), M. Wilson (London, United Kingdom), O. Usmani (London, United Kingdom), P. Shah (London, United Kingdom), M. Takata (London, United Kingdom). Late Breaking Abstract - Intra-alveolar neutrophil-derived microvesicles: a biomarker of COPD severity. 4921
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