Inhalation of hydrogen gas attenuate airway inflammation and oxidative stress in allergic asthmatic mice

J. Zhang (Shanghai, China)

Source: International Congress 2017 – Techniques to treat asthma

Session: Techniques to treat asthma
Session type: Thematic Poster
Number: 3565

Disease area: Airway diseases

Abstract

Inhalation of hydrogen gas attenuate airway inflammation and oxidative stress in allergic asthmatic mice

OBJECTIVE:

Oxidative stress was considered an important mechanism in the pathogenesis of asthma. Hydrogen (H₂) has been demonstrated to function as a novel antioxidant and exert therapeutic antioxidant activity in a number of diseases and the function of this nontoxic gas in asthma was unclear. This study aims to examine the effect of H₂ inhalation on the pathophysiology of a mice model of asthma.

METHODS:

A ovalbumin (OVA)-induced asthmatic mice model received inhalation of high concentration H₂ for 60 min once a day for 7 consecutive days after OVA or PBS challenge respectively. Lung function, morphology and goblet cell hyperplasia were assessed. BALF, serum and lung tissue were collected for biochemical assay.

RESULTS:

Inhalation of H₂ abrogated ovalbumin-induced the increase in lung resistance. The asthmatic mice showed severe inflammatory infiltration and goblet cell hyperplasia which were reversed by H₂ inhalation. H₂ inhalation reduced significantly the number of total cells, eosinophils and lymphocytes in bronchial alveolar lavage fluid. Increased level of IL-4, IL-13, TNF-α and CXCL15 in the BALF and IL-4 in the serum were decreased significantly after inhalation. H₂ inhalation markedly upregulated the activity of decreased superoxide dismutase and significantly attenuated the increased level of malondialdehyde and myeloperoxidase.

CONCLUSIONS:

H₂ inhalation improves lung function and protects established airway inflammation in the allergic asthmatic mice model which may be associated with the inhibition of oxidative stress process. This study provides a potential alternative therapeutic method for the clinical management of asthma.

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J. Zhang (Shanghai, China). Inhalation of hydrogen gas attenuate airway inflammation and oxidative stress in allergic asthmatic mice. 3565

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