The recent publication of all age, multi-ethnic reference values for spirometry potentially represent a great advance. However, these reference equations require local validation. To our knowledge, there are no pediatric spirometric reference values published in Argentina, and very few studies have been conducted in Latin America. Our goal was to compare the pediatric spirometric values from our pediatric Argentinian population with values from GLI-2012. The children were recruited from randomly selected schools and kindergartens from Trelew. Parents completed a written informed consent. Spirometry was performed following American Thoracic Society (ATS) guidelines for preschoolers and schoolchildren by the same technician on all occasions. We recruited a total of 2072 healthy children (50.92% males) for the conformation of our reference equations, using the LMS (Lambda-Mu-Sigma) method in the R 3.3.2 software. Table 1 compares the measured values in our population with predicted GLI values stratified by gender and by equation. There were no statistically significant differences except for the FEV1 in females using the “other/mixed” equation. This study shows that the “caucasian” GLI-2012 equation is appropriate for use in children in our Argentinian population, while the “other/mixed” equation frequently used in our country may not be appropriate.