Source: Annual Congress 2001 - Molecular and cellular pathology of asthma
Disease area: Airway diseases

Abstract

Asthma exacerbations are often associated with rhinovirus infections. The asthma-specific characteristics of lower airway inflammation following rhinovirus infection is still under debate [Fraenkel, AJRCCM 1995;151:879]. We adressed this issue by examining the difference in RV16-induced bronchial inflammation between 13 atopic asthmatics (FEV₁>= 70% pred., PC₂₀[lte]4.3 mg/ml) and 12 non-atopic non-asthmatics (FEV₁>=80% pred., PC₂₀>=13.5 mg/ml), who participated in a parallel study. Bronchial biopsies were taken 2 days before and 6 days after RV16-inoculation. Immunoperoxidase stained cells (EG2, elastase, AA1, CD3) in the lamina propria were automatically counted (KS400, Zeiss, cells/0.1 mm²), and cell numbers were log-transformed before analysis. Asthmatics had a significantly higher number of EG₂, AA1, and CD3 cells as compared to normals, at both timepoints (p<0.05). After infection, none of the cell numbers had changed significantly in either group. However, the increase in CD3 cells in the asthmatics was significantly larger compared with a decrease in normals (geom. mean ±] SEM: asthma: pre: 85.3±]0.24, post: 114.6±]0.18, normal: pre: 53.0±]0.20, post: 42.3±]0.22, p<0.05). We conclude that bronchial mucosal T cell-mediated response to rhinovirus cold is enhanced in subjects with mild asthma as compared with normals. This suggests that T cells play an asthma-specific role in rhinovirus-induced airway inflammation.
This abstract was funded by: Stichting Astma Bestrijding and Netherlands Asthma Foundation.

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J. de Kluijver, K. Grunberg, W. A. A. M. van Schadewijk, J. K. Sont, C. R. Dick, K. F. Rabe, J. H. J. M. van Krieken, P. J. Sterk (Leiden, Nijmegen, The Netherlands; Wisconsin, United States Of America). Difference between normals and asthmatics in bronchial inflammatory infiltrate following experimental rhinovirus 16 (RV16) infection in vivo . Eur Respir J 2001; 16: Suppl. 31, 3571

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