Source: Annual Congress 2001 - Molecular and cellular pathology of asthma
Disease area: Airway diseases

Abstract

The superoxide radical and its potent byproducts appear to play a major role in the microbicidal activity of phagocytic cells and inflammatory cells. Human neutrophils and eosinophils are known to generate superoxide anion. However, it has not been fully investigated whether human basophils could generate superoxide anion in response to various stimuli. We studied superoxide anion generation from human basophils induced by FcεRI or FcγRII crosslinking as well as PMA and/or ionophore A 23187. Highly purified basophils (purity>95%) were obtained by culturing peripheral blood stem cell-rich mononuclear cells with IL-3, followed by negative selection using immunomagnetic beads. Superoxide anion generation was measured by SOD-inhibitable reduction of ferricytochrome C. PMA (10ng/ml) significantly induced superoxide anion generation (4.37±]0.76 vs. control; 0.45±]0.37 nmol/l, p =0.0003). Ionophore A23187 (1mM) generated a smaller amount of superoxide anion (1.95±]0.59) than PMA (10ng/ml). Crosslinking of FcεRI by anti-FcεRIα chain antibody also induced superoxide anion generation (2.95±]0.73, p =0.005 ). On the other hand, crosslinking of FcγRII by anti-FcγRII antibody alone could not induce superoxide anion generation. Interestingly, co-stimulation of FcγRII and fibrinogen (Mac-1 ligand) could induce superoxide anion generation. These findings suggest that human basophils are capable of generating superoxide anion through Fc receptor stimulation, which is associated with allergic inflammation.

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N. Hamada, Y. Tanimoto, K. Takao, M. Fujii, M. Harada (Okayama, Japan). Human basophils can generate superoxide anion in response to Fc receptor stimulation. Eur Respir J 2001; 16: Suppl. 31, 3569

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