Expression of c-inhibitor of apoptosis protein-2 in the sputum from asthmatic patients
F. Bureau, G. Seumois, F. Jaspar, C. Desmet, R. Louis, P. Lekeux (Liege, Belgium)
Source: Annual Congress 2001 - Molecular and cellular pathology of asthma
Session: Molecular and cellular pathology of asthma
Session type: Poster Discussion
Number: 3567
Disease area: Airway diseases
Abstract Background: A defect in eosinophil apoptosis contributes to the development of chronic inflammation of the airways in asthma. However, the anti-apoptotic mechanisms which are responsible for the delayed eosinophil apoptosis are not completely identified.Objective: We sought to determine whether the inhibitor of apoptosis proteins c-IAP1, c-IAP2, and XIAP, are involved in increasing eosinophil survival in asthma.Methods: Sputum was obtained from 18 asthmatic patients with various disease severity and 12 nonatopic control subjects. Differential cell counts were performed and whole-cell extracts were analysed for c-IAP1, c-IAP2, and XIAP expression by immunoblots.Results: c-IAP1, c-IAP2, and XIAP were undetectable in the sputum from control subjects. c-IAP1 and XIAP were never detected in the sputum from asthmatics. Conversely, large amounts of c-IAP2 were found in sputum samples from asthmatic patients. To determine whether c-IAP2 was present in eosinophils, the correlation between the percentage of eosinophils and the level of c-IAP2 expression, as determined by photodensitometry, was calculated. This regression analysis revealed a strong positive correlation (r = 0.85) between the two variables, with very important levels of c-IAP2 expression in sputum samples containing more than 50% eosinophils.Conclusion: These results suggest that c-IAP2 expression results in increased eosinophil survival in asthma.
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F. Bureau, G. Seumois, F. Jaspar, C. Desmet, R. Louis, P. Lekeux (Liege, Belgium). Expression of c-inhibitor of apoptosis protein-2 in the sputum from asthmatic patients. Eur Respir J 2001; 16: Suppl. 31, 3567
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