Source: Annual Congress 2001 - Molecular and cellular pathology of asthma
Disease area: Airway diseases

Abstract

Allergic rhinitis is characterised by increased lumenal levels of ECP. However, granule proteins in the lumen may not necessarily reflect degranulation in the underlying tissue. Hence, little is known regarding eosinophil degranulation in the target tissue. By quantitative transmission electron microscopy (TEM) we have recently identified piecemeal degranulation (PMD) as a major mode of eosinophil degranulation in human tissues (AJRCCM 2000:161;2074-). PMD reflects gradual release of granule proteins. We recently developed a method for quantification of PMD by TEM analysis. The present study quantifies PMD in the nasal mucosa of patients with seasonal allergic rhinitis. Biopsies and nasal lavage samples were collected before and during the Swedish birch pollen season. Natural allergen exposure increased nasal symptoms, lavage fluid ECP, and number of tissue eosinophils (p<0.001). Although PMD occurred prior to the season (37±]3.7% granules that were altered due to PMD) natural pollen exposure increased PMD (87±]1.8 % altered granules, p<0.01). Thus, this study demonstrates, for the first time, that PMD occurs already under non-symptomatic baseline conditions and that this mode of degranulation is increased by natural allergen exposure. We suggest that PMD determinations are essential for assessment of eosinophil involvement in disease processes.

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J. S. Erjefält, C. Ahlström-Emanuelsson, M. Anderssson, L. Greiff, C. G. A. Persson (Lund, Sweden). Occurrence of piecemeal degranulation of human eosinophils before and after natural allergen exposure. Eur Respir J 2001; 16: Suppl. 31, 3562

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