Source: Annual Congress 2001 - Molecular and cellular pathology of asthma
Disease area: Airway diseases

Abstract

The aim of this study was to investigate the expression of the chemokines receptors CCR3, CCR4 and CCR8 in the airway mucosa of asthmatics after allergen challenge. Immunohistochemical analysis was performed on bronchial biopsies obtained from 6 atopic asthmatics 24 hours after an allergen challenge that elicited a dual asthmatic response and from 6 atopic asthmatics 24 hours after a sham inhalation challenge. We found that, as compared with sham challenged asthmatics, allergen challenged asthmatics had a significantly higher number of CD3+ve cells expressing CCR4 (median 3410 interquartile range [2790-4480]cells/mm² vs 390 [261-606]cells/mm²; P<0.01) and, to a lesser extent, of CD3+ve cells expressing CCR8 (982 [762-1103]cells/mm² vs 276 [238-293]cells/mm²; P<0.01) in the airway mucosa. Furthermore, a positive correlation was found between the number of CCR8+ cells in the airway mucosa of allergen challenged asthmatics and the maximal decrease in FEV1 during the late asthmatic response. CCR3 was detectable only on eosinophils, but not on CD3+ve cells, infiltrating the airway mucosa of allergen challenged asthmatics. In conclusion these data support the role of the chemokine receptors, particularly CCR8, in the pathogenesis of allergen induced late asthmatic responses.
*AP and PP-B equally contributed to this work.

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A. Papi, P. Panina-Bordignon, M. Mariani, P. Di Lucia, G. L. Casoni, C. Bellettato, C. Buonsanti, D. Miotto, C. E. Mapp, A. Villa, G. Arrigoni, L. M. Fabbri, F. Senigaglia (Ferrara, Milano, Italy). Allergen challenge increases the number of airway Th2 cells expressing C-C chemokine receptors CCR4 and CCR8. Eur Respir J 2001; 16: Suppl. 31, 3553

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