Source: Annual Congress 2001 - Drug resistant TB
Disease area: Respiratory infections

Abstract

In the last decades, tuberculosis (TB) has reemerged as one of the leading causes of Human death (some 3 million deaths annually (Ginsberg, 1998). Russia is a country where the disease took a impending scale.
It was detected very high level of primary (9.5%) and acquired (21.1%) resistance to rifampicine. For these reasons rapid susceptibility testing of M.tuberculosis has become critical for therapy selection and for prevention spread of resistant organisms. Classical methods needs 2-3 months for drug-resistance determination.
Resistance to rifampicine, a key component of antituberculosis therapy, is conferred by rpoB gene. More than 90% of rifr M. tuberculosis strains from different countries appear to harbor specific point mutation located in (505-531) region of rpoB gene.
For detection of other mutation which play the additional role in emergence of rifr we PCR amplify the 202-351 region of rpoB gene.
All the rifr strains with no mutation in 505-531 codons were used for PCR-SSCP analysis of 202-260 codons. Most PCR patterns from these region have changed mobility in SSCP analysis which point to existence of mutation in this region.
No PCR patterns with changed mobility were detected in control group.
So we can conclude that for rapid detection of rifr by PCR-SSCP it is necessary to use simultaneously two regions of rpoB gene: 505-531 and 202-260 codons.

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S. I. Tatkov, E. M. Kozhina, O. U. Smirnova, Y. V. Tumanov, E. V. Medvedeva, A. P. Duntau (Koltsovo, Novosibirsk, Russia). Additional region of rpoB (202-260) for rapid detection of rifampicine resistant of M. tuberculosis by PCR-SSCP. Eur Respir J 2001; 16: Suppl. 31, 3468

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