Source: Annual Congress 2001 - Interstitial lung disease: BAL in diagnosis and management
Disease area: Interstitial lung diseases

Abstract

Induced sputum (IS) has been recently used to investigate pulmonary inflammation in patients with interstitial lung disease. In 8 patients with hypersensitivity pneumonitis (HP, 6 male, mean age 54yr±]10) we assessed the cellular profile of IS and compare it to that of BAL. As a control group, we studied 10 healthy subjects (HS, 3 male, mean age 31yr±]7). IS was performed at least 2 days before bronchoscopy. In IS, the total cell count (mean ±] SD), the percentage of lymphocytes and eosinophils were higher in HP than HS (7.7 x105/mL±] 5 vs 3.6x105/mL±]2, p< 0.02; 4.3%±]3 vs 1.2%±]2, p< 0.02; 4.3%±] 8% vs 0.1%±]0.3, p< 0.02). Additionally, in BAL fluid the total cell count, the percentage of lymphocytes and eosinophils were higher in HP than HS ( 3.6x105/mL±]6 vs 1.4x105/mL±]0.4, p< 0.01; 55.6 %±]26 vs 2.6 ±]3%, p< 0.01; 1.2%±]1 vs 0.2%±]0.2, p<0.04), while the percentage of macrophages was lower in HP than HS (38%±]20 vs 95%±]3, p<0.01). Comparison between different samples showed higher total cell count (p<0.01) and lower lymphocytes (p<0.001) in IS than BAL from patients with HP, but no difference in eosinophils. Our results show that in HP patients, IS sample, as well as BAL fluid, contain more lymphocytes and eosinophils when compared to that of HS and suggest that IS is not an alternative tool to BAL to assess pulmonary inflammation.

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R. D'Ippolito, A. Chetta, A. Foresi, A. Castagnaro, M. Aiello, E. Marangio, G. F. Consigli, D. Olivieri (Parma, Milan, Italy). Induced sputum and BAL from patients with hypersensitivity pneumonitis. Eur Respir J 2001; 16: Suppl. 31, 3420

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