Source: Annual Congress 2001 - Interstitial lung disease: BAL in diagnosis and management
Disease area: Interstitial lung diseases

Abstract

The aim of this study was to explore induced sputum (IS) cellular composition and T-lymphocyte subpopulation and to compare it with bronchoalveolar lavage fluid (BALF) in patients with sarcoidosis.
Material and method: Twenty patients with sarcoidosis (8 male, 12 female), median age 48 yr were studied. IS was performed after inhalation of 1 to 3,5% saline with an ultrasonic nebulizer (DeVilbis 2000). BAL was performed within 10 days of IS. May- Grunwald-Giemsa stained preps were differentially counted and T-lymphocytes subsets were analyzed by flow activated cell shorter (FACS). Results are shown in Table (x±]SD).IS CD4/CD8 ratio was significantly correlated with BALF CD4/CD8 ratio (r=0.7, p=0.001).

Sample	AMF	LYMF	NEUTR	CD4+	CD8+	CD4+/CD8+
BALF	65,3±]16	29±]16	4,9±]3,1	40,9±]12,3	14,2±]7,7	4,5±]3,7
IS	40,9 ±]18,2	26,5±]15,3	32,4±]17,1	37,2±]11,7	8,4±]4,3	5,6±]3,2
p	p<0.0001	p=0.28	p<0.0001	p=0.25	p=0.001	p=0.1

Conclusion. Our results suggest that induced sputum is an effective noninvasive technique to identify inflammation in sarcoidosis.

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Citations should be made in the following way:
I. Tsiligianni, D. Kyriakou, N. Tzanakis, G. Chrysofakis, N. Siafakas, D. Bouros (Heraklion, Greece). Comparison of induced sputum and bronchoalveolar lavage cell differential in patients with sarcoidosis. Eur Respir J 2001; 16: Suppl. 31, 3419

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