Apoptosis and CD95 receptor in the main cells of induced sputum (IS) in bronchial asthma (BA) and chronic obstructive bronchitis (COB) patients
V. A. Nevzorova, E. N. Konovalova, T. N. Surovenco, S. A. Pazych (Vladivostok, Russia)
Source: Annual Congress 2001 - Inflammation and genetics in airway disease
Session: Inflammation and genetics in airway disease
Session type: Oral Presentation
Number: 3389
Disease area: Airway diseases
Abstract The purpose of investigation was to study an apoptosis index (AI) and content of a CD95-receptor in main cells of IS in BA and COB patients in developing disease. By using an ultrasonic nebulizer induced sputum was taken after 3-5% NaCl solution was inhaled. Immunofluorescence method with monoclonal antibody was used for the identification of a CD95- receptor. As a result the reduction of AI was proven in BA and COB patients in the period of disease excerebration compared with the control (16,3±]1,9%, 40,6±]4,3%, 78±]0,86% respectively, p<0,05). Using corticosteroids (CS) in BA patients and antibiotic therapy in COB patients in the period of disease excerebration an AI was increased to 41,5±]1,6% è 66,7±]3,9%, 41,5±]1,6%, 66,7±]3,9% respectively (p<0,05). An AI was up also during remission in BA patients and in COB patients but not reached the control data (p<0,05). The being of a CD95-receptor in main cells of IS in BA and COB patients was estimated in all groups. The quantity of CD95- positive cells was higher in content of a CD95-receptor in main cells of IS in BA and COB patients and did not differ in COB patients compared with the control (24,6±]3,5%, 5,2±]1,8%, 4,8±]2,3% respectively).Using CS in BA patients follows to decreasing a CD95-receptor to 17,3±]2,1% (p<0,05). In case of COB a CD95- positive cells did not depend on a period of a disease (p<0,05). Therefore, by BA and COB in the period of disease excerebration an AI was proven in main cells of IS but in using antiphlogistic therapy was increased.The quantity of CD95-positive cells was increased in case of BA patients in the period of disease excerebration and not changed of COB.
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V. A. Nevzorova, E. N. Konovalova, T. N. Surovenco, S. A. Pazych (Vladivostok, Russia). Apoptosis and CD95 receptor in the main cells of induced sputum (IS) in bronchial asthma (BA) and chronic obstructive bronchitis (COB) patients. Eur Respir J 2001; 16: Suppl. 31, 3389
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