Source: Annual Congress 2001 - Inflammation and genetics in airway disease
Disease area: Airway diseases

Abstract

Up-regulation of IL-8 and its receptors CXCR1 & 2 is considered to be important to the recruitment of airway mucosal neutrophils in exacerbations of COPD. However, data to support this hypothesis are lacking. We have applied in situ hybridisation to bronchial biopsy tissue to quantify cells expressing mRNA for either CXCR1 or 2 and immunohistochemistry to identify neutrophils by neutrophil elastase. The biopsies were obtained from 16 patients intubated for a severe exacerbation of COPD (Int-COPD), 9 subjects with mild stable COPD (Stable-COPD) and 18 normal healthy non-smokers (NH-NS). The table shows the numbers of positive cells as median (range) per mm2 of subepithelium:

Patient group	CXCR1+ cells	CXCR2+ cells	Neutrophils
NH-NS (n=18)	27.8 (0-577)	14.6 (0-248)	0 (0-12)
Stable-COPD (n=9)	33.2 (5-245)	12.3 (5-126)	0.6 (0-7)
Int-COPD (n=16)	79.1 (12-344)*	69.8 (0-591)*	68.2 (0-594)*

* p<0.01 compared to NH-NS and the stable-COPD groups (Mann-Whitney U test).
The intensity of CXCR2 mRNA expression within the cells were stronger than that of CXCR1.There was a significant association between the numbers of neutrophils and CXCR2+ cells (r=0.79; p<0.01; Spearman rank correlation) which was not seen with CXCR1+ cells (r=0.47; p=0.08). In conclusion, significantly increased numbers of neutrophils are recruited to the airway mucosa in severe exacerbations of COPD and this is associated with an up-regulation of both CXCR1 & 2.
Funding: Glaxo-Wellcome R&D, UK.

Rating:

Share or cite this content

Citations should be made in the following way:
Y. S. Qiu, J. Zhu, V. Bandi, A. E. Fraire, K. K. Guntapalli, P. K. Jeffery (London, United Kingdom; Texas, Boston, United States Of America). Neutrophil chemokine receptor (CXCR1 and 2) gene expression and biopsy neutrophilia in severe exacerbations of chronic obstructive pulmonary disease (COPD). Eur Respir J 2001; 16: Suppl. 31, 3386

Member's Comments

No comment yet.

Related content which might interest you: Neutrophils and neutrophil chemokine receptor (CXCR1 and 2) gene expression in severe exacerbations of COPD and asthma Source: Eur Respir J 2001; 18: Suppl. 33, 501s Year: 2001 Enhanced expression of proinflammatory cytokine (IL-18) and its receptor in patients with chronic obstructive pulmonary disease (COPD) Source: Eur Respir J 2006; 28: Suppl. 50, 551s Year: 2006 Enhanced expression of IL-18 receptor but not soluble form of IL18R in chronic obstructive pulmonary disease (COPD) Source: Annual Congress 2011 - COPD: clinical studies and animal cell models Year: 2011 Levels of CC-chemokines (MCP-1, MIP-1α, MIP-1β) in induced sputum of patients with chronic obstructive pulmonary disease and patients with chronic bronchitis Source: Eur Respir J 2001; 18: Suppl. 33, 194s Year: 2001 Blood cytokine expression in patients with chronic obstructive airway diseases (COAD) Source: International Congress 2017 – Systemic and airway biomarkers Year: 2017 Endobronchial biopsy neutrophilia, IL-8, ENA-78 and neutrophil chemokine receptor (CXCR1 & CXCR2) gene expression in severe exacerbations of COPD Source: Eur Respir J 2002; 20: Suppl. 38, 377s Year: 2002 Increased lung apoptosis and expression of ceramide in subjects with chronic obstructive pulmonary disease (COPD) Source: Annual Congress 2009 - COPD: basic science and clinical studies Year: 2009 Expression profiling of Th17 associated molecules in bronchoalveolar cells from patients with chronic obstructive pulmonary disease (COPD) Source: Annual Congress 2011 - COPD: clinical studies and animal cell models Year: 2011 Altered expression of TLR4 and of beta 2 defensin in chronic obstructive pulmonary disease Source: Eur Respir J 2006; 28: Suppl. 50, 549s Year: 2006 Altered expression of TLR4 and of beta defensin 2 in chronic obstructive pulmonary disease Source: Eur Respir J 2007; 30: Suppl. 51, 213s Year: 2007 The expression of MMP2, 9 and TIMP1 mRNA in bronchoalveolar cells of patients with chronic obstructive pulmonary disease (COPD) and bronchial asthma Source: Annual Congress 2009 - New results in molecular pathology and functional genomics of non-neoplastic lung diseases Year: 2009 Platelets activation during chronic obstructive pulmonary disease (COPD) exacerbation Source: Eur Respir J 2002; 20: Suppl. 38, 89s Year: 2002 Periostin levels in chronic obstructive pulmonary disease (COPD) Source: International Congress 2018 – Biomarkers for evaluating COPD Year: 2018 Angiopoietins in sputum supernatant of patients with chronic obstructive pulmonary disease (COPD) Source: Annual Congress 2010 - Induced sputum in relation: a fresh look on phenotyping and monitoring Year: 2010 M2 macrophage marker CD163, CD204 and CD206 expression on alveolar macrophages in the lung of patients with chronic obstructive pulmonary Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 Airway neutrophilia in COPD is not associated with increased neutrophil survival Source: Eur Respir J 2006; 28: 1163-1169 Year: 2006 Suppressed pulmonary expression of leptin in lipopolysaccharide-induced acute and chronic lung inflammation Source: Annual Congress 2006 - Novel pathogenic aspects of acute lung injury and acute respiratory distress syndrome (ARDS) Year: 2006 Apoptosis and CD95 receptor in the main cells of induced sputum (IS) in bronchial asthma (BA) and chronic obstructive bronchitis (COB) patients Source: Eur Respir J 2001; 18: Suppl. 33, 502s Year: 2001 Evidence of mast-cell activation in a subset of patients with eosinophilic chronic obstructive pulmonary disease Source: Eur Respir J 2002; 20: 325-331 Year: 2002 Endobronchial biopsy neutrophilia, IL-8, ENA-78 and neutrophil chemokine receptor (CXCR1 and CXCR2) gene expression in severe exacerbations of asthma Source: Eur Respir J 2003; 22: Suppl. 45, 588s Year: 2003