Neutrophils and neutrophil chemokine receptor (CXCR1 and 2) gene expression in severe exacerbations of COPD and asthma
J. Zhu, Y. S. Oiu, V. Bandi, A. E. Fraire, K. K. Guntupalli, P. K. Jeffery (London, United Kingdom; Texas, Massachusetts, United States Of America)
Source: Annual Congress 2001 - Inflammation and genetics in airway disease
Session: Inflammation and genetics in airway disease
Session type: Oral Presentation
Number: 3385
Disease area: Airway diseases
Abstract Exacerbations in either chronic obstructive pulmonary disease (COPD) or asthma are associated with an increased recruitment of airway tissue neutrophils compared with their stable phase. The present study determines the relative numbers of neutrophils recruited to the airway mucosa and compares the numbers of cells expressing the genes for CXCR1 & 2 in acute severe exacerbations of COPD and asthma. In situ hybridisation (ISH) was applied to tissue sections of biopsies obtained from 18 normal healthy non-smokers (NH-NS), 16 patients intubated for severe COPD (Int-COPD) and 28 intubated for a severe exacerbation of their asthma (Int-asthma). The numbers of neutrophil-elastase+ and CXCR1 and 2 mRNA+ cells were counted and the results are shown below as median (M) and range (R) per mm2 of subepithelial tissue:
Patient group CXCR-1+ cells CXCR-2+ cells Neutrophils NH-NS (n=18) 28 (0-577) 15 (0-248) 0 (0-11) Int-COPD (n=16) 79(12-344)* 70(0-591)* 68 (0-594)* Int-asthma (n=28) 96 (9-664)* 277(0-1060)*3 51 (0-898)*
*P<0.01 compared to the NH-NS group. 3 P<0.01 compared to the CXCR-1of int-asthma and CXCR-2 of int-COPD (Mann-Whitney U-test). In conclusion, neutrophils and both CXCR 1 & 2 are increased in severe exacerbations of COPD and asthma. CXCR-2 is particularly strongly expressed in intubated asthma, greatly in excess of the increased numbers of neutrophils. We gratefully acknowledge the support of Glaxo-Wellcome Research & Development and the British Lung Foundation, UK.
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J. Zhu, Y. S. Oiu, V. Bandi, A. E. Fraire, K. K. Guntupalli, P. K. Jeffery (London, United Kingdom; Texas, Massachusetts, United States Of America). Neutrophils and neutrophil chemokine receptor (CXCR1 and 2) gene expression in severe exacerbations of COPD and asthma. Eur Respir J 2001; 16: Suppl. 31, 3385
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