Source: Annual Congress 2001 - Inflammatory mechanisms of COPD
Disease area: Airway diseases

Abstract

COPD posses remarkable place in the structure of post-Chernobyl pathology in liquidators especially in those with maximal exposure during 1986. Flow cytometric analysis (FCA) of BAL leukocytes were performed in 62 patients exposed in 1986-87. Leukocytes were identified by CD45 common leukocyte antigen (AG) expression, three-part differential was used for granulocytes (Grl), lymphocytes (Lph) and monocytes/macrophages discrimination. Antibody panel included CD3, CD4, CD8, CD10, CD13, CD14, CD16, CD19, CD33, CD45, CD56, HLADR. Additional staining of intracellular matrix was performed with anti-cytokeratin. Squamous metaplasia and cell atypia of bronchial epithelium in the cases of atrophic and sclerotic changes were associated with high cytokeratin expression. Grl exhibited high CD45 expression. Prevalence of CD8+56+ cytotoxic cells was shown among the Lph with high HLADR expression while CD4+ subset size was significantly lower than in peripheral blood (PhB) samples. Quantitative studies of AG expression showed similarity of average figures with the PhB for most of the differentiation AGs while HLADR expression on CD8+ cells and monocyte/macrophage cells was elevated. Somatic mutations study does not reveal variant Lph lacking T-cell receptor. Chronic progradient course of disease showed lower signs of cytotoxic cell activation and Grl counts. Increase of circulating immune complexes concentrations were not associated with significant activation of BAL B-cells. Differences in subset organisation revealed by FCA could indicate activated cell tropism to lung tissue and also be the markers of an involvement of cytotoxic mechanisms in the formation of late post-irradiation bronchopulmonary disorders.

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D. A. Bazyka, V. A. Sushko, L. I. Kozachuk (Kiev, Ukraine). Flow cytometric analysis of bronchoalveolar lavage leukocytes in COPD-patients after radiation exposure. Eur Respir J 2001; 16: Suppl. 31, 2119

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