Source: Annual Congress 2001 - Inflammatory mechanisms of COPD
Disease area: Airway diseases

Abstract

The present study aimed at assessing the intracellular cytokine production by T cells in different airway diseases. Seven patients with allergic asthma (1 f / 6 m; age 51 yr; FEV₁, 79 %pred) and 8 patients with COPD (1 f / 7 m; age 64 yr; FEV₁, 61 %pred) underwent sputum induction according to a standard protocol. Intracellular cytokine production of sputum lymphocytes with and without stimulation by PMA / ionomycin was determined by flow cytometry. Target parameters were IFN-g, IL-2, IL-4 and IL-5 in CD8-positive (TS) and CD8-negative (TH) T cells. In the asthmatic subjects without corticosteroids, basal production of IFN-g, IL-2, IL-4 and IL-5 by total T cells was considerably higher than in the asthmatic patients with steroids or patients with COPD or healthy control subjects. There were no differences in basal production between asthmatic subjects with steroids and those with COPD. Production of IL-4 by TH cells after stimulation was higher in COPD than in asthma (p<0.05); conversely, the production of IFN-g by stimulated TS cells was elevated in asthma. These data suggest that, irrespective of the difficulties regarding the definition of comparable patients' groups, TH1/TH2 cytokine production by sputum T lymphocytes appears not to be a function of the presence of allergic vs. non-allergic airway disease only. Supported by LVA Freie und Hansestadt Hamburg, and Gemeinschaftspraxis Dr. Kramer und Kollegen, Geesthacht

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Citations should be made in the following way:
D. Loppow, M. Böttcher, G. Gercken, H. Magnussen, R. A. Jörres (Grosshansdorf, Geesthacht, Hamburg, Germany). Flow cytometric assessment of sputum lymphocyte cytokine profiles in patients with asthma and COPD. Eur Respir J 2001; 16: Suppl. 31, 2118

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