Identification and quantification of NF-kB transcription factor in chronic obstructive pulmonary disease
A. Di Stefano, G. Caramori, A. Capelli, M. Lusuardi, B. Balbi, K. Fan Chung, P. Balbo, C. F. Donner, P. J. Barnes, I. M. Adcock (Veruno, Pavia, Novara, Italy; London, United Kingdom)
Source: Annual Congress 2001 - Inflammatory mechanisms of COPD
Session: Inflammatory mechanisms of COPD
Session type: Oral Presentation
Number: 2116
Disease area: Airway diseases
Abstract Activation of the transcription factor NF-kB is involved in the regulation of many inducible genes that encode for a number of inflammatory proteins. To investigate the relationship between nuclear expression of p65 protein (an NF-kB subunit) and inflammatory cell infiltration in smokers with COPD, we examined bronchial biopsies from 41 subjects: 9 with severe fixed airflow obstruction (34±]4 FEV1% pred.), 12 with mild/moderate fixed airflow obstruction (68±]3 FEV1% pred.), 14 non-obstructed smokers (109±]4 FEV1% pred.) and 6 control non smokers (113±]6 FEV1% pred.) with normal lung function. Nuclear p65 protein (p65nu+), CD3+, CD4+ and CD8+ cells in the sub-epithelium were analysed using frozen bronchial biopsies by immunohistochemistry. p65 protein expression was also quantified by Western blot (WB) analysis of bronchial biopsies in mild/moderate COPD and control groups. Patients with severe COPD had lower numbers [median (range)] of p65nu+ (0 (0-29) vs 22 (0-136) vs 18 (0-88) vs 23 (0-82) cells/mm2), CD3+ (309 (115-663) vs 599 (441-1067) vs 539 (198-1139) vs 383 (177-582) cells/mm2) and CD8+ (182 (59-310) vs 292 (176-520) vs 334 (100-676) vs 154 (101-286) cells/mm2) cells compared with mild/moderate COPD (p<0.005) and control smokers (p<0.03) and control non-smokers (p<0.05). p65 expression in the bronchial biopsies was increased in mild/moderate COPD and control smokers (0.80 (0.3-1.0) vs 1.1 (0-2.0) vs 0.25 (0.03-0.6) p65/actin, p<0.04) in comparison with control non smokers. NF-kB expression may be involved in the pathogenesis of mild/moderate COPD.
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A. Di Stefano, G. Caramori, A. Capelli, M. Lusuardi, B. Balbi, K. Fan Chung, P. Balbo, C. F. Donner, P. J. Barnes, I. M. Adcock (Veruno, Pavia, Novara, Italy; London, United Kingdom). Identification and quantification of NF-kB transcription factor in chronic obstructive pulmonary disease. Eur Respir J 2001; 16: Suppl. 31, 2116
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