Source: Annual Congress 2001 - Inflammatory mechanisms of COPD
Disease area: Airway diseases

Abstract

It has been shown that in the sputum samples obtained during three consecutive 10 min inhalation periods, with increasing saline concentration, differ in their cellular composition with a significant fall of neutrophils. The aim of the study was to determine the possible difference in the induced sputum cellular composition between samples obtained during three consecutive 5 min. inhalation period with the same saline concentration (4.5%). Twenty asthmatics (mean age 49 ±] 13 years; FEV1 92 ±] 20 % of predicted) and 10 COPD subjects (mean age 64 ±] 15 years; FEV1 69 ±] 23 % of predicted) produced sputum during and after three consecutive 5 min inhalation periods. The three samples were analysed separately for total and differential cell count. The mean percentage of neutrophils decreased during sputum induction in asthmatics (58.6 ±] 15; 49.8.±] 21; 47.8 ±] 20 % ) and COPD subjects (71.0 ±] 11; 65.1±] 15; 61.9 ±] 16 %), but the decrease did not reach the significance in the two groups (ANOVA and Bonferroni t Test). To conclude, the separate analysis of induced sputum from three consecutive 5 min inhalation periods, demonstrated a non statistically significant decrease of neutrophils in asthmatic and COPD subjects.

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A. Spanevello, G. B. Migliori, B. Chizzoni, A. Roberti, E. Stocco, M. Neri (Cassano delle Murge, Tradate, Italy). Changes in sputum composition during 15 minutes of sputum induction in asthmatic and COPD subjects. Eur Respir J 2001; 16: Suppl. 31, 2113

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