Source: International Congress 2016 – Pulmonary hypertension and pulmonary embolism: from the bench to the bedside
Disease area: Pulmonary vascular diseases

Abstract

Pulmonary Arterial Hypertension (PAH; OMIM 178600) is a rare disease characterized by pulmonary vascular resistance increase and right heart failure. The most implicated gene is BMPR2, with a reduced expression in PASMC cells. The aim was to analyze the BMPR2 gene expression, in different cell cycle phases, in B-Lymphocytes from patients and controls.B-lymphocytes from patients and controls were aisled from peripheral blood by Ficoll method. These B cells were immortalized using Epstein-Barr Virus and grown in appropriate conditions. Lymphoblastoid cell line were analyzed by flow cytometry and separated based on the cell cycle phase by sorting. With patients and controls RNA, we realized a RT-PCR and, finally, we analyzed BMPR2 gene expression with TaqMan assay.After analysis of BMPR2 gene expression by qPCR using TaqMan assay, we detected expression for BMPR2 gene in Lymphoblastoid cell line from patients and controls. The normalized gene expression results in BMPR2 gene, using GADPH as reference gene, shows a greater expression levels in G₂/M phase, followed by G₀/G₁ phase and, finally, in S phase. However, sorting cell analysis by flow cytometry show changes in ploidy from patients relative to controls. The results of BMPR2 gene expression in patients, will tell us if there are any expression differences between patients and controls. Genotype-phenotype correlation hasn´t been made.Although the availability of a tissue that express BMPR2 like lung is a problem, we have confidence in that it is a good method to determine the gene expression. G₂/M phase show a greater expression level to BMPR2 gene, but we don´t found expression differences between patients and controls.


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Guillermo Pousada (Vigo, Spain), Guillermo Pousada, Mauro Lago, Sonia Prado-López, Adolfo Baloira, Rubén Varela-Calviño, Diana Valverde. Analysis of BMPR2 gene expression in B-lymphocytes of pulmonary arterial hypertension patients. Eur Respir J 2016; 48: Suppl. 60, 2486

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