Source: Annual Congress 2002 - Interstitial lung disease
Disease area: Interstitial lung diseases

Abstract

We have previously shown that alveolar macrophages (AM) of IPF patients exhibit elevated levels of bioactive TNF-α in comparison to normal controls. Additionally, there is only a 2-fold increase in IL-10 protein relative to a 5-fold increase in IL-10 mRNA suggesting that the level of IL-10 produced is unable to maintain the homeostatic regulation of TNF-α in IPF. We are currently examining the role of the IL-10 receptor (IL-10R) in IPF. Altered levels of IL-10R or aberrant signalling may be linked to altered IL-10 levels in IPF. The IL-10R complex is composed of two subunits, IL-10R1 and IL-10R2. The level of IL-10R1 and IL-10R2 mRNA was determined by semi-quantitative RT-PCR of total RNA from AM of IPF (n=7) patients and normal (n=5) controls. AM were isolated by adherence from bronchoalveolar lavage fluid and cultured in vitro +/- 10μg/ml lipopolysaccharide (LPS). PCR products were quantified by densitometry as OD units/mm². The results are expressed as the median ratio of
IL-10R/GAPDH.

	UNSTIMULATED	LPS STIMULATED
Normal IL-10R1	0.781	0.754
IPF IL-10R1	0.587	0.592
Normal IL-10R2	1.098	1.045
IPF IL-10R2	1.038	1.072

The level of IL-10R1 mRNA is lower in IPF patients than in normal controls but there is no difference in the level of IL-10R2 mRNA. We are currently examining the level of IL-10R protein by flow cytometry and alterations in signalling. This work is supported by the British Lung Foundation.

Rating:

Share or cite this content

Citations should be made in the following way:
H. A. Whittington, A. B. Millar (Westbury-on-Trym, United Kingdom). The role of IL-10 receptors in aberrant homeostatic control of TNF-α in idiopathic pulmonary fibrosis (IPF). Eur Respir J 2002; 20: Suppl. 38, 3753

Member's Comments

No comment yet.

Related content which might interest you: Elevated integrin ?5 expression in idiopathic pulmonary fibrosis (IPF) derived fibroblasts is linked to paracrine TNF-a signaling Source: International Congress 2017 – Cellular signalling pathways in pulmonary fibrosis Year: 2017 RhoA signalling influences cyclin D1 expression and associated fibroblast turnover: implications for idiopathic pulmonary fibrosis (IPF) Source: Annual Congress 2006 - Lung vascular and parenchymal remodeling Year: 2006 Does IFN-g slow progression of idiopathic pulmonary fibrosis (IPF)? Source: Eur Respir J 2005; 26: Suppl. 49, 335s Year: 2005 The role of chemokines and cytokines in lung fibrosis Source: Eur Respir Rev 2008; 17: 151-156 Year: 2008 Cykine/receptor inflammatory profile in different types of idiopathic intestitial pneumonias (IIPs). Key role of Fas Ligand recruvation in idiopathic pulmonary fibrosis (IPF) Source: Annual Congress 2010 - Biomarkers in diffuse parenchymal lung disease Year: 2010 M2 polarized alveolar macrophages in pulmonary fibrosis Source: Annual Congress 2005 - Pulmonary hypertension and fibrosis: novel mechanisms Year: 2005 Expression of mRNA of selected inflammatory markers in BALf cells from patients with sarcoidosis and idiopathic pulmonary fibrosis (IPF) Source: International Congress 2017 – IPF: from the bench to the bedside Year: 2017 Difference in immunological aspects of inflammation in idiopathic pulmonary fibrosis (IPF) and exogenous toxic alveolitis (ETA) Source: Eur Respir J 2003; 22: Suppl. 45, 121s Year: 2003 Low telomerase expression in alveolar epithelial plays a role in the pathogenesis of idiopathic pulmonary fibrosis Source: Annual Congress 2008 - Management of idiopathic pulmonary fibrosis: what can we learn from the pathophysiology? Year: 2008 Activated lymphoid follicles, sign of an immune response, are present in early idiopathic pulmonary fibrosis (IPF) and increase with disease progression. Source: Virtual Congress 2021 – Biomarkers and e-health in idiopathic interstitial pneumonia Year: 2021 Interleukin-17 (IL-17) contributes to silica-induced alveolitis but is not essential in fibrosis development Source: Eur Respir J 2006; 28: Suppl. 50, 675s Year: 2006 Enhanced expression of proinflammatory cytokine (IL-18) and its receptor in patients with chronic obstructive pulmonary disease (COPD) Source: Eur Respir J 2006; 28: Suppl. 50, 551s Year: 2006 The matrix-fibroblast interplay contributes to HIF-1 alpha pathway activation: a possible therapeutic target in idiopathic pulmonary fibrosis (IPF) Source: Virtual Congress 2020 – Elucidating the mechanisms underlying lung repair and exploring therapeutic strategies in vitro Year: 2020 Dysregulation of the TGF-beta system in idiopathic pulmonary fibrosis Source: Eur Respir J 2004; 24: Suppl. 48, 10s Year: 2004 The circulating metabolome in idiopathic pulmonary fibrosis (IPF) Source: International Congress 2019 – The evolving field of idiopathic interstitial pneumonia Year: 2019 Enhanced expression of IL-18 receptor but not soluble form of IL18R in chronic obstructive pulmonary disease (COPD) Source: Annual Congress 2011 - COPD: clinical studies and animal cell models Year: 2011 Cytokine profiles in idiopathic pulmonary fibrosis suggest an important role for TGF-β and IL-10 Source: Eur Respir J 2003; 22: 69-76 Year: 2003 PD-L1 expression in patients with idiopathic pulmonary fibrosis. Source: Virtual Congress 2021 – Biomarkers and e-health in idiopathic interstitial pneumonia Year: 2021 Expression profile of Th1/Th2 transcription factors T-bet and GATA-3 in sarcoidosis, idiopathic pulmonary fibrosis and chronic obstructive pulmonary disease Source: Annual Congress 2010 - Usefulness of bronchoalveolar lavage in diffuse parenchymal lung disease Year: 2010 High mobility group box protein 1 (HMGB1) production in highly vascularized alveolar lesions causes acute exacerbation in idiopathic pulmonary fibrosis Source: Annual Congress 2005 - Progresses in lung fibrosis Year: 2005