Source: Annual Congress 2002 - Interstitial lung disease
Disease area: Interstitial lung diseases

Abstract

We have observed a downregulation of CCR5 expression in alveolar cells and an important role played by CC-chemokines (MIP 1alfa and MIP-1beta) in fibrotic stages of sarcoidosis (1). To evaluate if there is a similar involvement in IPF, 35 subjects were studied. Eighteen non smoker control subjects and 17 patients affected by IPF underwent BAL. CCR5 expression was evaluated in alveolar macrophages and lymphocytes; MIP-1alfa, MIP-1beta and MCP-1 levels were measured with ELISA in BAL fluid concentrated 10 fold. The table shows the results as median and range:

	MIP-1α pg/ml	MIP-1β pg/ml	MCP-1 pg/ml
Controls NS	1.2 (0.0 - 3.8)	3.4 (0.3 - 6.8)	4.6 (0.5 - 119.4)
IPF	1.6 (1.0 - 11.1)*	6.2 (1.3 - 96.0)**	60.1 (16.7 - 251.3)***

p = 0.036 , ** p = 0.0015, *** p < 0.0001 vs controls.
A significant decrease of CCR5 expression was observed in lymphocytes (5.0% vs 20.5% p < 0.002) but not in macrophages ( 2.3% vs 2.5% p = NS) in IPF compared with controls. In IPF as in sarcoidosis, CC-chemokines are involved in the inflammatory reaction of the lung. The CCR5 expression, decreased in lymphocytes, is in agreement with previous observations of a Th2 immunity reaction in this interstitial lung disease.
1) Capelli A. et al. Am J Respir Crit Care Med 2002;165:236-241.

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Citations should be made in the following way:
A. Capelli, A. Di Stefano, M. Lusuardi, I. Gnemmi, C. F. Donner (Veruno, Italy). CCR5 expression and CC-chemokine levels in broncho-alveolar lavage (BAL) fluid of patients with idiopathic pulmonary fibrosis (IPF). Eur Respir J 2002; 20: Suppl. 38, 3751

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