e-learning
resources
Amsterdam 2015
Wednesday, 30.09.2015
Advances from translational research in respiratory infections
Login
Search all ERS
e-learning
resources
Disease Areas
Airways Diseases
Interstitial Lung Diseases
Respiratory Critical Care
Respiratory Infections
Paediatric Respiratory Diseases
Pulmonary Vascular Diseases
Sleep and Breathing Disorders
Thoracic Oncology
Events
International Congress
Courses
Webinars
Conferences
Research Seminars
Journal Clubs
Publications
Breathe
Monograph
ERJ
ERJ Open Research
ERR
European Lung White Book
Handbook Series
Guidelines
All ERS guidelines
e-learning
CME Online
Case reports
Short Videos
SpirXpert
Procedure Videos
CME tests
Reference Database of Respiratory Sounds
Radiology Image Challenge
Brief tobacco interventions
EU Projects
VALUE-Dx
ERN-LUNG
ECRAID
UNITE4TB
Disease Areas
Events
Publications
Guidelines
e-learning
EU Projects
Login
Search
Neutrophils are immuno-modulatory in rhinovirus infections
Francesca Tang (Glebe, Australia), Francesca Tang, Philip Hansbro, Janette Burgess, Katherine Baines, Brian Oliver
Source:
International Congress 2015 – Advances from translational research in respiratory infections
Session:
Advances from translational research in respiratory infections
Session type:
Poster Discussion
Number:
5036
Disease area:
Airway diseases, Respiratory infections
Abstract
Background:
Neutrophils are important in controlling bacterial infections however; their role in viral infections remains unclear. Previously, we found that neutrophils respond to viral mimetics but not replication competent rhinovirus (RV).
Aim:
To investigate if neutrophils are activated when exposed to with RV-infected monocytes. We hypothesised that neutrophils would become activated in the presence of another activated cell.
Methods:
Primary human neutrophils and monocytes were co-cultured +/-RV16 or LPS, with either direct contact or separated by transwells (n=5-13). RV16 or LPS stimulated monocytes were exposed to killed neutrophils, neutrophil membrane or soluble neutrophil intracellular components (n=4-6). IL-6 and CXCL8 mRNA and protein levels were measured by qPCR and ELISA at 24h (n=4-7).
Results:
Constitutive IL-6 and CXCL8 release and LPS and RV-induced IL-6 from monocytes was inhibited when cultured with neutrophils. Direct contact with neutrophils was required for this inhibitory effect as it did not occur with transwell separation. Killed neutrophils inhibited constitutive and RV-induced monocyte derived IL-6 and CXCL8 compared to monocytes alone. Neutrophil intracellular components inhibited monocyte derived IL-6 and CXCL8 constitutively and with stimulation compared to monocytes alone, except LPS induced CXCL8. RV induced monocyte IL-6 and CXCL8 mRNA was reduced when monocytes were cultured with neutrophil intracellular components.
Conclusion:
We describe a novel anti-inflammatory property of neutrophils dependent on cell contact and neutrophil mediator release which may be important in the pathogenesis of viral induced asthma exacerbations.
Rating:
You must
login
to grade this presentation.
Share or cite this content
Citations should be made in the following way:
Francesca Tang (Glebe, Australia), Francesca Tang, Philip Hansbro, Janette Burgess, Katherine Baines, Brian Oliver. Neutrophils are immuno-modulatory in rhinovirus infections. Eur Respir J 2015; 46: Suppl. 59, 5036
You must
login
to share this Presentation/Article on Twitter, Facebook, LinkedIn or by email.
Member's Comments
No comment yet.
You must
Login
to comment this presentation.
Related content which might interest you:
Late Breaking Abstract - Implications of treatable traits and treatment choices on exacerbation risk in moderate-severe asthma
Management of Severe Asthma in Pediatric Patients by an Interdisciplinary Team in a Public Hospital Setting.
Respiratory sequelae of preterm birth across the life span
Related content which might interest you:
Induction of galectin-9 production by viral infection in the lung
Source: International Congress 2015 – The multiple faces of host defense
Year: 2015
IP-10 is selectively produced in the airways upon respiratory virus infection
Source: Annual Congress 2013 –Macrophages, mononuclear cells and viral infection
Year: 2013
Investigation of the innate antiviral response to respiratory syncytial virus
Source: International Congress 2015 – Paediatric pulmonary infections: mechanisms and treatment
Year: 2015
Role of the immunoregulatory microRNA miR-146a in the innate immune response to rhinovirus infection
Source: Annual Congress 2013 –Viral infections: what is new?
Year: 2013
LATE-BREAKING ABSTRACT: The use of in vitro Human Airway Epithelia for the development of novel antivirals against human rhinoviruses (HRV)
Source: International Congress 2014 – Respiratory infections in children
Year: 2014
Type I interferons and IL-15 regulate antiviral cellular responses to rhinovirus infection
Source: Annual Congress 2013 –Viral infections: what is new?
Year: 2013
Human T-lymphocytes are affected by notch ligands in vitro
Source: International Congress 2014 – Novel approaches to human studies in allergy and asthma
Year: 2014
Modulation of PD-1 and PD-L1 expression in response to acute viral infection of human lung tissue
Source: International Congress 2014 – Cell biology 2014
Year: 2014
Clinical impact of rhinovirus infections in infancy
Source: Annual Congress 2013 –The burden of respiratory infections in children
Year: 2013
LATE-BREAKING ABSTRACT: Inhibition of Pim1 kinase augments the anti-viral response to rhinovirus infection.
Source: International Congress 2014 – COPD exacerbations
Year: 2014
Neutrophil elastase-activated host defence peptide prodrugs kill
pseudomonas aeruginosa
in a cystic fibrosis lung milieu
Source: International Congress 2014 – New studies into pulmonary proteases and antiproteases
Year: 2014
Glycosylation studies of G-protein of BA genotype of group B human respiratory syncytial virus in mammalian cells
Source: International Congress 2015 – Respiratory infections: from basic science to clinical issues
Year: 2015
Mapping the cellular source and role of IL-22 in murine lung infections
Source: International Congress 2015 – Viral infections: basic research and clinical perspective
Year: 2015
The role of neutrophils in viral clearance during respiratory syncytial virus (RSV) disease
Source: Annual Congress 2013 –Pulmonary infection in children: diagnosis and long-term effects
Year: 2013
A role for the WFDC protein, WAP2, in regulation of innate host defence in the lung
Source: International Congress 2014 – New studies into pulmonary proteases and antiproteases
Year: 2014
A human lung explant model for the study of antiviral drugs
Source: Annual Congress 2013 –Interesting research questions
Year: 2013
Hyperoxia impairs the inflammatory response of alveolar macrophages to multiple Toll-like receptor ligands associated with bacterial infection
Source: International Congress 2014 – New insights into monocyte and macrophage biology
Year: 2014
Modulation of neutrophil functionalities by indacaterol and their relevance for chronic lung diseases
Source: International Congress 2014 – Translational studies in COPD
Year: 2014
An innate defence role for BPIFA1/SPLUNC1 against influenza-A virus infection
Source: International Congress 2015 – The multiple faces of host defense
Year: 2015
Innate immune response to respiratory syncytial virus and human rhinovirus in infants: Similarities and differences its effect on disease severity
Source: International Congress 2014 – Virus-triggered lower respiratory disease in children
Year: 2014
We use cookies on our website to give you the most relevant experience by remembering your preferences and repeat visits. By clicking "Accept", you consent to the use of the cookies.
Accept