Source: International Congress 2015 – Advances from translational research in respiratory infections
Disease area: Airway diseases

Abstract

Introduction: Respiratory viruses are a major cause of exacerbations for patients with chronic respiratory disease. Many studies have investigated the incidence of viruses in patients experiencing exacerbation using traditional sampling methods which are invasive, difficult to collect, and are unsuitable for repetitive measurements.Aim: To utilise the disposable electret filters used during routine spirometry testing as a virus collection system.Methods: Patients with COPD, asthma and bronchiectasis were recruited during their routine outpatient visit during a local influenza epidemic. They completed spirometry and provided a nasal washing or sputum sample. We looked for four common viruses (human rhinovirus, respiratory syncytial virus, influenza A and influenza B) in the secretion sample and in exhaled breath collected in electret spirometry filters, using RT-PCR.Results: 53 participants were recruited (COPD n=13; asthma n=17; bronchiectasis n=13) and 10 healthy volunteers (n=10). Virus was identified in the filters in 56% of participants, and of these, 81% had the same virus type in sputum/nasal washing samples. 77% of participants with COPD, 88% with asthma, and 92% with bronchiectasis, compared with 70% of healthy controls tested viral positive in at least one sample.Conclusion: Virus was detected more frequently in patients with chronic respiratory disease than healthy controls. Virus identified in electret filters correlated well with virus identified in sputum/nasal lavage. Detection and identification of virus obtained from exhaled breath collected in the electret filters may be a novel way to assess viral incidence in large populations or as part of routine clinical management.


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Citations should be made in the following way:
Alicia Mitchell (Naremburn, Australia), Alicia Mitchell, Francesca Tang, Qi Ge, Lucy Morgan, Brian Oliver. Respiratory viruses can be isolated and identified from exhaled breath. Eur Respir J 2015; 46: Suppl. 59, 5033

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