Source: International Congress 2015 – Advances from translational research in respiratory infections
Disease area: Airway diseases, Respiratory infections

Abstract

Objective: the identification of oropharyngeal streptococci species and analysis of genetic determinants of antibiotic resistance in patients with asthma and COPD. Materials and methods: The multiplex real-time PCR was applied to investigate the representation of antimicrobial resistance genes (mef and ermB) and species diversity of streptococci in oropharyngeal swabs from 89 stable COPD patients and 51 asthma patients. Results and discussion: the vast majority of the streptococci identified were presented by streptococci viridans mitis group. In a stable period of the disease S. pneumoniae in oropharyngeal swabs was detected in 7.8% of patients with asthma and 6.74% of patients with COPD. A common feature of these groups was the tendency to severe disease and the presence of recurrent exacerbation episodes with a requirement of antibiotics. S. pyogenus was detected in 42.9% oropharyngeal swabs from COPD and asthma patients. Identified streptococci are a reservoir of the antimicrobial resistance genetic determinants (mef and ermB genes), which coding mechanisms of streptococci drug resistance to the effects of macrolides. Mef genes were found in 100% asthma and COPD patients swabs. ErmB genes were determined in 91% of patients with COPD swabs and in 82.4% of asthma patient swabs. The representation of these genes directly correlates with the frequency of exacerbations and a number of antimicrobial therapy episodes. For COPD patients the direct correlations between the representation of ermB gene with the severity of sputum production index and smoking index were found. Mef and ermB genes directly correlated with the frequency of exacerbations in patients with COPD.


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Citations should be made in the following way:
Sergey Fedosenko (Tomsk, Russian Federation), Sergey Fedosenko, Ludmila Ogorodova, Elena Ilina. Genetic determinants of oropharyngeal streptococci antimicrobial resistance in COPD patients and asthma sufferers. Eur Respir J 2015; 46: Suppl. 59, 5032

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