Source: International Congress 2015 – Advances from translational research in respiratory infections
Disease area: Airway diseases, Respiratory infections

Abstract

The recovery of Pseudomonas aeruginosa from respiratory samples of COPD patients has been associated with impaired lung function and recurrent exacerbations, partly due to strain-dependent factors such as biofilm formation.AIMS:To analyse the bronchial microbiome of COPD patients with positive cultures for P.aeruginosa associated with either colonization or infection, and differentiated according to biofilm production, both in stability and exacerbation.METHODS:Sputum samples with positive cultures for P.aeruginosa were obtained from 21 COPD patients during stability (n=18) and exacerbation (n=23), and analysed by 16S rRNA gene amplification and pyrosequencing. Biofilm-forming capacity of P. aeruginosa was examined using crystal violet in a static microtiter plate assay. Sputum samples were classified according to the biofilm-forming capacity of P.aeruginosa (low/high), assessing the differences between both groups, both in stability and exacerbation.RESULTS:No significant differences in biodiversity parameters (Shannon and Chao1) were found between low and high biofilm-forming groups. However, the bronchial microbiome of the high biofilm-forming group showed higher relative abundance of bacterial Operational Taxonomic Units (12 and 9 OTUs during stability and exacerbation, respectively), and represented up to 10% of the relative abundance in the sample.CONCLUSIONS:In COPD patients colonized or infected by P. aeruginosa, biofilm production was associated with an overgrowth of some bacterial OTUs, which attained a relative abundance of up to 10%. Accordingly, a biofilm environment undermined a change in the bronchial microbiome identifiable through 16srRNA analyses.


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Marian Garcia-Nuñez (Sabadell, Spain), Marian Garcia-Nuñez, Sara Marti, Carmen Puig, Vicente Perez-Brocal, Laura Millares, Fina Liñares, Eduard Monso. Influence of biofim on the bronchial microbiome in COPD patients colonized or infected by pseudomonas aeruginosa. Eur Respir J 2015; 46: Suppl. 59, 5028

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