Source: International Congress 2015 – Advances from translational research in respiratory infections
Disease area: Airway diseases

Abstract

BackgroundVitamin (vitD) deficiency has been associated with increased risk at COPD exacerbations and 2 RCTs showed that vitD supplementation reduces exacerbations in patients with vitD deficiency. In this study, we aimed to further investigate how vitD could influence COPD exacerbations by exploring effects on inflammation and bacterial clearance in response to cigarette smoke (CS). MethodsHuman THP-1 macrophages were stimulated with cigarette smoke extract (CSE) and/or the active form of vitD (1,25(OH)₂D). The release of inflammatory mediators as well as phagocytosis and oxidative burst were determined. VitD deficient or control mice were exposed to CS or ambient air for 6 weeks, followed by infection with nontypeable Haemophilus influenzae (NTHi). Bacterial loads and phagocytosis by inflammatory cells in the BAL fluid were assessed 16, 40 and 64h post-infection.ResultsPost-treatment with 1,25(OH)₂D significantly reduced the release of IL-8, TNF-a and MCP-1 by THP-1 macrophages compared to treatment with CSE alone. The CSE-induced defect in the phagocytic bacterial uptake and killing by oxidative burst was not affected by 1,25(OH)₂D. Smoking also resulted in an impaired phagocytosis capacity of alveolar macrophages and neutrophils obtained from the BAL fluid of mice, which was not affected by long-term vitD deficiency in vivo. Surprisingly, bacterial loads were significantly lower in the vitD deficient mice compared to control mice 16, 40 and 64h post-infection, irrespective of CS exposure and phagocytosis capacity. ConclusionOur results suggest that the beneficial effect of vitD on COPD exacerbations may not be mediated by bacterial killing, but by inhibitory effects on airway inflammation.


Rating:

Share or cite this content

Citations should be made in the following way:
Nele Heulens (Oplinter, Belgium), Nele Heulens, Hannelie Korf, Karen Maes, Elien De Smidt, Conny Gysemans, Ghislaine Gayan-Ramirez, Chantal Mathieu, Wim Janssens. LATE-BREAKING ABSTRACT: Effect of vitamin D on inflammatory and antibacterial responses to cigarette smoke. Eur Respir J 2015; 46: Suppl. 59, 5027

Member's Comments

No comment yet.

Related content which might interest you: The effect of cigarette smoke on key pathogens in COPD lung infection Source: International Congress 2014 – Pathogenesis and mechanisms of respiratory infections Year: 2014 LATE-BREAKING ABSTRACT: Andrographolide helps steroid-resistant cigarette smoke-induced lung injury model to regain steroid sensitivity Source: International Congress 2016 – Novel mechanisms and therapeutic targets in acute and chronic inflammatory disease Year: 2016 LATE-BREAKING ABSTRACT: Effect of vitamin D deficiency and vitamin supplementation on severity and exacerbation rate of asthma Source: International Congress 2014 – Clinical assessment of airway diseases Year: 2014 Cryptotanshinone attenuates airway Inflammation induced by cigarette smoke in mice Source: International Congress 2016 – New signalling pathways in COPD Year: 2016 Evaluation of vitamin D levels in COPD and its correlation with disease severity and frequency of exacerbations Source: International Congress 2014 – Mechanisms of COPD and inhalation injury Year: 2014 Is vitamin D deficiency a risk factor for COPD exacerbations? Source: Annual Congress 2013 –Environment and lifestyle: associations with respiratory disease Year: 2013 LATE-BREAKING ABSTRACT: The Guangzhou COPD exacerbation study: relationship of exacerbations with viral infections and soluble biomarkers Source: International Congress 2014 – Systemic biomarkers for airway diseases Year: 2014 Ceramide-1-phosphate attenuates cigarette smoke induce lung inflammation Source: Annual Congress 2013 –Cell biology and murine models of asthma and inflammation Year: 2013 The effect of Vitamin D deficiency on lung function in current smoker COPD patients Source: International Congress 2014 – Rationale behind respiratory drug development Year: 2014 The relationship between systemic inflammation and vitamin D levels in COPD Source: Annual Congress 2013 –Factors associated with outcomes in COPD Year: 2013 Differences in the polarization of the inflammatory response of patients with chronic obstructive pulmonary disease (COPD) secondary to smoking and to biomass smoke exposure Source: Annual Congress 2013 –COPD: new markers of diseases Year: 2013 Reduced airway expression of cytoprotective CC10 in COPD patients and mice exposed to cigarette smoke Source: Annual Congress 2013 –COPD: experimental biology Year: 2013 Monitoring catalase activity in COPD exacerbations Source: International Congress 2016 – Airway biomarkers Year: 2016 LATE-BREAKING ABSTRACT: Exacerbation phenotype directed corticosteroid therapy of COPD: Value of blood eosinophilia (primary results) Source: International Congress 2015 – The relevance of diagnostic and monitoring tools in clinical practice Year: 2015 Vitamin D deficiency and COPD exacerbations: Effect of vitamin D supplementation Source: International Congress 2015 – Novel treatments for cough, asthma, COPD and bronchiectasis Year: 2015 The effect of vitamin D therapy on airway reactivity and airway inflammation in asthmatic children Source: Annual Congress 2013 –Preschool wheeze Year: 2013 Chronic obstructive lung disease and vitamin D status: Relationship with lung function, inflammatory status and exacerbations Source: International Congress 2015 – Monitoring airway diseases with lung function tests Year: 2015 Impact of cigarette smoke exposure on host–bacterial pathogen interactions Source: Eur Respir J 2012; 39: 467-477 Year: 2012 The effect of smoking on the activity of inflammatory markers in patients with COPD Source: Annual Congress 2013 –Tobacco use in different countries: crisis to disease Year: 2013