Source: International Congress 2015 – New data on established treatments for asthma, COPD and bronchiectasis
Disease area: Airway diseases

Abstract

AIM: Chronic obstructive pulmonary disease (COPD) is an inflammatory lung disease mainly caused by tobacco smoke inhalation. We aimed to evaluate antiinflamatory and antioxidant effect of tiotropium bromide which is an anticholinergic bronchodilator in subacute cigarette exposure mice model.METHOD: Thirtyfive healthy 25-40 gram male balb-c mices were categorized into 3 groups: (A) control group (n=7), (B) exposed to cigarette smoke (CS) (n=18), and (C) exposed to CS treated with tiotropium group(n=10. After 5 weeks, tiotropium or saline was given to mices during two weeks by inhalation. Mices were anestetized, broncoalveolar lavage was performed and lung tissues removed. Interleukin-1b (IL1b), macrophage inflammatory protein-1 (MIP 1), tumor necrosis factor alpha (TNF a) measurements were evaluated in BAL fluid. Total oxidant and antioxsidant status (TAS-TOS) measurements were evaluated in lung tissue.RESULTS: We found that cigarette exposure significantly decreased TAS and increased TOS (p<0,001; p<0,001). TOS and TOS/TAS ratio was significantly improved by using tiotropium (p<0.05). Furthermore, mepenzolate significantly improved IL 1 b, TNF a and MIP 1 levels (p<0.05).


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Aysun Şengül (Kocaeli, Turkey), Aysun Sengul, Meyrem Ozkan, Ceyla Eraldemir, Leman Huseynova, Doga Ozsoy, Firuzan Akar. The antiinflammatory and antioxidant effect of tiotropium bromide in subacute cigarette exposure mouse model. Eur Respir J 2015; 46: Suppl. 59, 2964

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