Source: International Congress 2015 – Case series and clinical conundrums in TB
Disease area: Respiratory infections

Abstract

Introduction. Little information is available about the specific mode of action of cationic surfactants on MBT. However, since low concentrations are mycobacteriostatic, but not generally mycobactericide.The Goal of the study was to determine minimal tuberculocidal concentration of cationic surfactants.Material and methods. MBT cultures belonging to such genetic familiesas Beijing, Ural, Lam included in the study.We used the tissue-object method. The tissue-object contaminated MBT (10⁵-10⁶ colony-forming units (cfu) per 1 cm²) were immersed into solutions of cationic surfactants, 60 minutes later neutralization of remaining actions was carried out, then the inoculation on solid medium Lowenstein Jensen (L-J) was performed.Classification of results was made according to quantitative characteristics of MBT growth on medium.Results and discussion.The results show the resistance of MBT culture to 0,05% and 0,075% cationic surfactants solutions since the growth of typical cfu of MBT has been found. However after making use of these concentration was decrease some of number MBT cfu. This fact demonstrate tuberculostatic effect.Tuberculocidal effect was observed after exposure to 0,15% solution of the MBT culture of the Beijing genotype, to 0,22% solution of the Lam genotype and to 0,26% solution of the Ural genotype.Conclusion. The 0,26% of acting substance in solutions of cationic surfactants is minimal tuberculocidal concentration of cationic surfactants. MBT various genotypes showed different degrees of resistance to solutions of cationic substance. Ural genotype showed the greatest resistance, Lam followed. Beijing genotype showed the lowest resistance.


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Citations should be made in the following way:
Natalya Eremeeva (Yekaterinburg, Russian Federation), Natalya Eremeeva, Diana Vakhruscheva, Marionella Kravchenco. Minimal inhibiting concentration of cationic surfactants against various genotypes of mycobacterium tuberculosis (MBT). Eur Respir J 2015; 46: Suppl. 59, 2768

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