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Amsterdam 2015
Monday, 28.09.2015
Case series and clinical conundrums in TB
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The effectiveness of polymerase chain reaction in testing the removed portion of lung during surgery
Fatima Tashpulatova (Tashkent, Uzbekistan), Mirza Nigmatovich Tillashayhov, Z.A. Sayfiddinov, Z.H. Nuritdinov, Fatima Tashpulatova, Abduvahob Baboev
Source:
International Congress 2015 – Case series and clinical conundrums in TB
Session:
Case series and clinical conundrums in TB
Session type:
Thematic Poster Session
Number:
2767
Disease area:
Respiratory infections
Abstract
Objective:
to evaluate the efficiency of MTB detection in tissue samples taken during surgery (TSTDS) using real-time PCR.
Materials and Methods:
As a diagnostic material the removed portion of lungs of patients with various forms of pulmonary TB from thoracic surgery department were tested. A total of 49 (30 (61%) male and 19 (39%) female) patients were examined. Patient categories: 37 (76%) new cases and 12 (24%) recurrent cases. To compare the efficiencies all pathological materials were examined by microscopy (fluorescence method), as well as molecular - genetic method on GeneXpert MTB / RIF equipment.
Results:
The results showed high sensitivity and specificity of molecular - genetic method in MTB detection in TSTDS. In 33 out of 49 pathological materials the Xpert MTB / RIF test was positive. Sensitivity to rifampicin anti TB drug was in 18 (55%) cases and resistance in 15 (45%) cases. 18 patients with drug sensitivity were new cases of TB in 12 (67%) of them and recurrent cases in 6 (33%). 15 patients with drug resistance were new cases of TB in 12 (80%) and recurrent cases in 3 (20%) patients. The Xpert MTB / RIF test detected MTB in 12 (36%) patients with negative sputum smears, 4 (33%) of them were resistant forms of MBT. Test error was found in 7 (14%) patients. The average test time on GeneXpert apparatus was two hours per test.
Conclusions:
Molecular and genetic method based on real-time PCR reduces the detection time of MTB and the
rpoB
gene mutation in TSTDS, which contributes to the timely correction of treatment regimen of patients.Xpert MTB / RIF test can be used as an initial diagnostic test in analysis of TSTDS in various forms of pulmonary TB.
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Fatima Tashpulatova (Tashkent, Uzbekistan), Mirza Nigmatovich Tillashayhov, Z.A. Sayfiddinov, Z.H. Nuritdinov, Fatima Tashpulatova, Abduvahob Baboev. The effectiveness of polymerase chain reaction in testing the removed portion of lung during surgery. Eur Respir J 2015; 46: Suppl. 59, 2767
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