The role of secondary lymphoid tissue in airway obliteration in a heterotopic trachea transplant model
Jana Somers (Leuven, Belgium), Jana Somers, Elly Vandermeulen, Philippe Lemaitre, Hannelore Bellon, David Ruttens, Stijn Verleden, An Martens, Arne Neyrinck, Robin Vos, Geert Verleden, Bart Vanaudenaerde, Dirk Van Raemdonck
Source: International Congress 2015 – Pathogenic mechanisms of lung transplant complications: a focus on CLAD
Abstract Immune organization is the key element of transplant immunology in chronic rejection. In this study, we wanted to investigate to what extend secondary lymphoid tissue is involved in the granulation/fibroproliferation of the allograft. Heterotopic trachea transplantation of 2 allografts (C57BL6/J): one on the left (n=4) and one on the right side (n=4) was performed on the posterior back area of Balb/c mice (n=4). Prior to the tracheal implantation, the left inguinal lymph node was surgically removed. Grafts were harvested 22 days after transplantation and fixed in 4% paraformaldehyde, and Haematoxylin-Eosin staining was performed on 7µm-thick paraffin sections. The percentage of luminal obliteration was calculated. A decrease in luminal obliteration was observed between the standard allografts with inguinal lymph node present (82.8±12.4%) and the allografts with inguinal lymph node absent (42.9±5.8%)(p=0.027). In the vast majority of the group, we could clearly observe a more advanced lesion in the standard allografts compared to the allografts deprived of the inguinal lymph node . This study shows the importance of immune organization and especially secondary lymphoid tissue/organs in the development of true chronic allograft rejection and may need to be investigated in the different phenotypes of chronic lung allograft dysfunction to identify the true immunological chronic rejection in the human setting.
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Jana Somers (Leuven, Belgium), Jana Somers, Elly Vandermeulen, Philippe Lemaitre, Hannelore Bellon, David Ruttens, Stijn Verleden, An Martens, Arne Neyrinck, Robin Vos, Geert Verleden, Bart Vanaudenaerde, Dirk Van Raemdonck. The role of secondary lymphoid tissue in airway obliteration in a heterotopic trachea transplant model. Eur Respir J 2015; 46: Suppl. 59, 1789
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