Source: Annual Congress 2002 - Bronchial responsiveness in asthma and COPD
Disease area: Airway diseases

Abstract

It is well known that the β-adrenoceptor antagonist propranolol can induce airway obstruction in asthmatic patients. Propranolol has been shown to increase the sensitivity of airways to several contractile agents, both in vivo and in vitro. Initially, this potentiation was attributed to the β-adrenoceptor blocking effect of propranolol but recent studies indicate that other mechanisms are involved. The aim of this study was to further examine the mechanism behind the effects of propranolol on guinea pig airways, in vitro. Airway responsiveness to ovalbumin was determined in sensitised tracheal ring preparations 1h after propranolol treatment. Capsaicin treatment was used to evaluate a possible role of sensory neuropeptides in the responses. Cytosolic calcium transients in both adherent cells and cells in suspension were measured using a fura-2AM technique. In the preparations propranolol (10^-6 M) had no intrinsic effect, but significantly augmented the contractile response to ovalbumin with 70 % (p<0.01). Depletion of sensory nerves with capsaicin reduced the effect of propranolol. In ASM cells propranolol directly induced distinct calcium responses in several experiments. However, incubation with propranolol (10^-5 M) did not alter the subsequent calcium response to bradykinin (10^-9 M). These results indicate that propranolol enhance the hyperreactivity to ovalbumin in sensitised guinea pig airways in vitro. This effect was in part mediated by neuropeptides from sensory nerves. We also conclude that propranolol, by unknown mechanisms, is able to cause calcium transients in smooth muscle cells and could therefore directly influence ASM cell activity.

Rating:

Share or cite this content

Citations should be made in the following way:
S. E. V. Albinsson, M. K. L. Grenegård, E. G. Lindström (Linköping, Sweden). The β-adrenoceptor antagonist propranolol induced Ca²⁺ mobilisation and enhanced contractile responses in guinea pig airways, in vitro. Eur Respir J 2002; 20: Suppl. 38, 1207

Member's Comments

No comment yet.

Related content which might interest you: Antagonism by reproterol of adenosine-mediated tension responses in isolated guinea pig trachea Source: Eur Respir J 2003; 22: Suppl. 45, 106s Year: 2003 Endogenous PGE2 contributes to antigen-induced contractions of guinea pig trachea Source: Annual Congress 2011 - Models of disease and drug actions Year: 2011 Alpha-1L, but not alpha-1H, adrenoceptor antagonist prevents allergic bronchoconstriction in guinea pigs in vivo Source: Eur Respir J 2001; 18: Suppl. 33, 104s Year: 2001 The influence of epithelium on the effect of isoprenaline incubation in responsiveness of guinea-pig trachea to b-adrenergic agonist and antagonist Source: Annual Congress 2007 - Airway regulation, provocation and monitoring - III Year: 2007 Late asthmatic response is modulated by TRPA1 antagonists in ovalbumin-induced bronchoconstriction in anaesthetized guinea pigs Source: Annual Congress 2012 - New drug targets and pre-clinical models for respiratory diseases Year: 2012 PGD2 induces constriction of the peripheral guinea pig lung via the TP receptor Source: Annual Congress 2008 - Novel experimental approaches to understanding asthma and COPD Year: 2008 A novel TRPA1 antagonist inhibits ovalbumin-induced bronchoconstriction in guinea pig models Source: International Congress 2019 – Airway inflammation: pharmacological mechanisms and novel targets Year: 2019 Different patterns of mast cell activation and smooth muscle contractions by MRGPRX2 agonist and antigen in guinea pig trachea Source: Virtual Congress 2020 – New insight into the pathogenesis of chronic lung diseases: asthma, COPD and others Year: 2020 The cannabinoid receptor agonist, WIN 55,212-2, inhibits antigen-induced plasma extravasation in guinea pig airways Source: Annual Congress 2010 - Models of airways disease Year: 2010 Biphasic modulation of adenosine(AR)-induced bronchial responses during chronic caffeine treatment in guinea pigs Source: Eur Respir J 2004; 24: Suppl. 48, 130s Year: 2004 Discovery of competitive thromboxane receptor (TP) antagonism by diclofenac in guinea pig airway and vascular tissues Source: Annual Congress 2007 - Recent advances in airway smooth muscle biology Year: 2007 Potentiation effect of insulin on M3 muscarinic receptor agonist induced airway smooth muscle contraction Source: International Congress 2019 – Airway inflammation: pharmacological mechanisms and novel targets Year: 2019 Effects of pinacidil, a KATP channel opener, on proliferation of cultured rabbit airway smooth muscle cells induced by endothelin-1 Source: Eur Respir J 2004; 24: Suppl. 48, 80s Year: 2004 Zileuton abolishes an indomethacin-insensitive tone that develops in EFS contracted guinea pig tracheal smooth muscle Source: Eur Respir J 2003; 22: Suppl. 45, 105s Year: 2003 Ryanodine receptors decant internal Ca2+ store in human and bovine airway smooth muscle Source: Eur Respir J 2008; 32: 275-284 Year: 2008 The Rho-kinase inhibitor Y-27632 reduces cholinergic nerve-mediated contraction but not neurotransmitter release from guinea-pig isolated trachea Source: Eur Respir J 2004; 24: Suppl. 48, 97s Year: 2004 Development of bronchospasm in normal and sensitized rabbits: exogenous administration vs. endogenous liberation of constrictor agonists Source: Eur Respir J 2005; 26: Suppl. 49, 365s Year: 2005 Bronchoconstriction induced by prostaglandin D2 (PGD2) in guinea pig lung is enhanced by selective COX-2 inhibition Source: Eur Respir J 2006; 28: Suppl. 50, 759s Year: 2006 The influence of epithelium on the responsiveness of guinea-pigs trachea to β-adrenergic agonist and antagonist Source: Eur Respir J 2002; 20: Suppl. 38, 504s Year: 2002 Increased tracheal responsiveness to β-adrenergic agonist and antagonist in ovalbumin sensitized guinea-pigs Source: Eur Respir J 2004; 24: Suppl. 48, 588s Year: 2004