Source: Annual Congress 2002 - Isolated lung perfusion and lung cancer

Abstract

OBJECTIVE: Isolated lung perfusion (ILuP) with melphalan (MN) is superior to intravenous infusion in an experimental rat model for the treatment of pulmonary carcinoma and sarcoma metastases. We studied the influence of flow, perfusion time and concentration on melphalan lung levels in our rat model of isolated lung perfusion.
METHODS: To Determine the level at which the lung is saturated by MN, ILuP was performed in WAG/Rij rats during 30 minutes by a single pass system with 0.5 mg (n=5), 0.05 mg (n=5), 0.01 mg (n=5) and 0.005 mg (n=5) of MN. In a second experiment, the influence of flow, perfusion time and concentration was analyzed using the dose beneath saturation found in the first experiment. Lung perfusion was performed during 30 minutes with a flow of 0.5 mL/min (n=4), during 60 minutes with a flow of 0.5 mL/min (n=4) and during 30 minutes with a flow of 1.0 mL/min (n=4). For comparison of melphalan lung levels the unpaired Student's T-test was applied. Statistical significance was reached at p<0,05.
RESULTS: Melphalan lung levels of rats perfused with 0.5 mg (89.7±]47mg/g) and 0.05 mg (25±]0.3 mg/g) were significantly higher compared to the lower doses (0.01mg: 0.6±]0.3mg/g; 0.005mg: 1.4±]0.2mg/g). Perfusions performed with 0.5 mg were at a saturated level. Therefore 0.05 mg was chosen as the dose for the second experiment. No significant differences were seen in lung levels of MN between the groups with variation in perfusion time, flow and concentration.
CONCLUSION: since no influence of flow, perfusion time and concentration was observed with a dose of 0.05 mg of MN, the only determinant of final lung MN levels is the dose administered. Consequently, MN can be given as a dose in the perfusion circuit or a concentration by single-pass perfusion.

Rating:

Share or cite this content

Citations should be made in the following way:
S. Romijn, J. M. H. Hendriks, B. P. Van Putte, P. E. Y. Van Schil (Edegem, Belgium). Flow, perfusion time and the concentration of perfusate do not influence the final concentration of melphalan in the lung after isolated lung perfusion in rats. Eur Respir J 2002; 20: Suppl. 38, 1149

Member's Comments

No comment yet.

Related content which might interest you: Dialysate-based perfusate of low viscosity is suitable for normothermic ex vivo lung perfusion Source: Annual Congress 2010 - Pre-operative evaluation and post-operative problems after lung transplantation Year: 2010 Perfused lung volume to thoracic gas volume ratio (perfused lung fraction) in subjects with COPD and controls Source: Annual Congress 2010 - State of the art imaging Year: 2010 Acute increase in pulmonary blood flow elevates airway responsiveness in isolated perfused rat lungs Source: Annual Congress 2008 - Respiratory pathophysiology from cell and animal research Year: 2008 Pulmonary intravascular volume can be used for dose calculation in isolated lung perfusion Source: Annual Congress 2005 - New insights into prognosis in thoracic surgical oncology Year: 2005 Survival and pharmacokinetics of gemcitabine in a rat model of isolated lung perfusion Source: Eur Respir J 2002; 20: Suppl. 38, 179s Year: 2002 Correlation between differential lung flow measured by planar perfusion images and lung volume measured by perfusion SPECT Source: Annual Congress 2007 - Imaging of pulmonary vascularisation and illustrated overview of lung pathology Year: 2007 Neutrophil extracellular traps in ex vivo lung perfusion perfusate predict the clinical outcome of lung transplant recipients Source: Eur Respir J, 53 (4) 1801736; 10.1183/13993003.01736-2018 Year: 2019 Changes in lung volume with PEEP following surfactant depletion in rabbits assessed by helium wash-out technique and synchrotron imaging Source: Annual Congress 2010 - Lung imaging Year: 2010 Impaired TF following exercise is related to reduced lung capillary blood volume but is attenuated by an increase in the lung membrane diffusion capacity Source: Eur Respir J 2003; 22: Suppl. 45, 408s Year: 2003 Inflammative responses during reperfusion after cold preservation in the isolated and ventilated perfused rat lung Source: Eur Respir J 2006; 28: Suppl. 50, 779s Year: 2006 Ex-vivo donor lung perfusion Source: ERS Course 2016 Year: 2016 Addition of neutrophil elastase inhibitor to flushing solution decreases reperfusion injury in rat lung transplantation Source: Eur Respir J 2005; 26: Suppl. 49, 176s Year: 2005 Extravascular lung fluid accumulation does not immediately follow the increased capillary permeability during exposure to high altitude (HA) Source: Annual Congress 2004 - Clinical physiology: airways and vessels Year: 2004 Impact of elevated pulmonary blood flow and capillary pressure on lung responsiveness Source: Annual Congress 2009 - Experimental approaches in animals and modelling in humans Year: 2009 Effects of prone position on the amount of overdistension and collapsed lung tissue and pulmonary blood flow, in an experimental model of acute lung injury Source: Annual Congress 2009 - Quality control in lung function and new developments Year: 2009 Ventilation and perfusion changes following bronchoscopic lung volume reduction Source: Annual Congress 2009 - Treatment beyond inhalers Year: 2009 Advantage of retrograde flush perfusion with low potassium dextran solution for preservation in lung transplantation Source: Eur Respir J 2001; 18: Suppl. 33, 178s Year: 2001 Experimental retrograde single-pass isolated lung perfusion (SP-ILP) affords homogeneous drug distribution to the lung Source: Eur Respir J 2001; 18: Suppl. 33, 14s Year: 2001