Source: Annual Congress 2002 - Interstitial lung disorders: markers of disease activity and severity
Disease area: Interstitial lung diseases

Abstract

Background: Sarcoidosis is thought to be a type-1 cytokine-mediated disorder. Few data are available on the relevance of Th1/Th2 type cytokines to the course of the disease, which may be important for the fibrogenic potential of Th2 type cytokines.
Objective: To determine whether there is an association between the cytokine patterns of patients with sarcoidosis and different stages of disease.
Methods: Using three-colour flow cytometry we evaluated the intracellular expression of interferon (IFN)γ and interleukin (IL)-4 in unstimulated CD4+ and CD8+ T lymphocytes from bronchoalveolar lavage (BAL) of 17 untreated patients (7 men, mean age 48.18 ±] 4.1 yr, nonsmokers) stratified according to radiographic appearance. 5 patients had type I chest radiograph (bilateral hilar lymphadenopathy), 6 patients had type II (hilar lymphadenopathy plus reticulonodular parenchymal infiltrates), 4 patients had type III (parenchimal infiltration without hilar adenopathy), 2 patients had type IV (fibrotic disease).
Results: All patients showed spontaneous intracellular expression of cytokines IFNγ and IL-4 in CD4+ as well as CD8+ BAL T lymphocytes; this was most pronounced for CD4+ cells (%CD4/IFNγ+ = 21.3±]3; %CD4/IL-4+ = 9.49±]1.6; %CD8/IFNγ+ = 1.38±]0.43; %CD8/IL-4+ = 0.69±]0.09). There were no differences between IFNγ and IL-4 producing cells among CD4+ and CD8+ T lymphocytes from patients with different stages of disease.
Conclusions: Data shows that a spontaneous cytokine expression can be observed in CD4+ as well as CD8+ BAL T lymphocytes from patients with sarcoidosis. Our failure to demonstrate a shift toward a type-2 phenotype in the later stages of the disease may be explained by the low number of patients with evidence of end stage lung fibrosis.

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Citations should be made in the following way:
M. Maria, C. Antonella, C. Massimo, P. Alberto (Parma, Milan, Italy). T-cell cytokine pattern in bronchoalveolar lavage fluid of patients affected by different stages of pulmonary sarcoidosis. Eur Respir J 2002; 20: Suppl. 38, 1118

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