Detection of Mycobacterium tuberculosis DNA from peripheral blood in patients with HIV-seronegative and new cases of smear-positive pulmonary tuberculosis by polymerase chain reaction
N. Taci, A. S. Yurdakul, I. Ceyhan, B. M. Berktas, M. Ogretensoy (Ankara, Turkey)
Source: Annual Congress 2002 - Diagnosis of tuberculosis
Session: Diagnosis of tuberculosis
Session type: Thematic Poster Session
Number: 1069
Disease area: Respiratory infections
Abstract Tuberculosis is still one of the most important cause of mortality and morbidity in many countries and there is a need new methods for accurate and rapid diagnosis of tuberculosis. To determine the sensitivity and specificity of polymerase chain reaction (PCR) method, we have evaluated M.tuberculosis DNA in peripheral blood samples with PCR technique in adult patients with HIV-seronegative and new cases of smear-positive pulmonary tuberculosis. We investigated the relationship between characteristic features of the patients, radiological disseminity, presence of cavity, body-mass index, level of serum albumin, the time of symptoms and presence of additional disease and PCR positivity. 40 patients (33 male and 7 female; mean age 37.8±]14.1) and 20 healthy control subjects (13 male and 7 female; mean age 35.6±]7.3) were enrolled in this study. PCR was positive in 16(40%) blood samples of patients with pulmonary tuberculosis and the others (24(60%)) were negative. None of the healthy controls had positive PCR results. The overall sensitivity, specificity and accuracy of the PCR assay for a diagnosis of tuberculosis was 40 %, 100 % and 60 %, respectively. We found the positive correlation between PCR positivity and the degree of sputum asido resistant bacteria (ARB) positivity (r= 0.462, p:0.003), disseminated lesions in chest X-ray (r=0.690, p:0.001), in patients who have cavitary lesions (r=0.903, p:0.001). We conclude that the detection of M.tuberculosis DNA from peripheral blood by PCR technique is useful for the rapid diagnosis of tuberculosis patients with HIV-seronegative.
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N. Taci, A. S. Yurdakul, I. Ceyhan, B. M. Berktas, M. Ogretensoy (Ankara, Turkey). Detection of Mycobacterium tuberculosis DNA from peripheral blood in patients with HIV-seronegative and new cases of smear-positive pulmonary tuberculosis by polymerase chain reaction. Eur Respir J 2002; 20: Suppl. 38, 1069
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