Source: Annual Congress 2002 - The role of inflammatory markers in children with asthma
Disease area: Airway diseases, Paediatric lung diseases

Abstract

8-Isoprostane, a marker of oxidative stress, is increased in exhaled breath condensate (EBC) of adults with asthma (Montuschi P. et al. Am. J. Respir. Crit. Care Med. 1999; 160:216-220). EBC is a non-invasive method of sampling secretions from the airways. The aim of this study was to quantify lung oxidative stress in children with asthma as reflected by exhaled 8-isoprostane levels. We also measured exhaled nitric oxide (NO), a marker of airway inflammation, and exhaled prostaglandin (PG) E₂, since impaired production of this eicosanoid has been proposed to contribute to the pathogenesis of asthma. Twelve healthy children (3/9 M/F, age 9±]0.5 yr, FEV₁ 96.3±]3.5% pred) and 30 children with mild to moderate persistent asthma (20/10 M/F, age 11±]0.6 yr, FEV₁ 89.9±]2.6% pred) treated with inhaled corticosteroids (average dose 300 mg/day) were studied. Exhaled 8-isoprostane and PGE₂ were measured by specific radioimmunossays developed in our laboratory which were validated by high performance liquid chromatography. Exhaled NO was measured with the NIOX system (Aerocrine, Sweden). Exhaled 8-isoprostane was increased in children with asthma (47.2±]2.3 pg/ml; P<0.008; 95% confidence interval [CI], 42.4-52.0) compared to healthy children (34.2±]4.5 pg/ml; 95% CI, 24.3-44.0), whereas exhaled PGE₂ concentrations were similar in the two groups (51.5±]4.0 pg/ml vs 46.8±]5.3 pg/ml, respectively). Children with asthma (37.8±]6.6 ppb, P<0.04; 95% CI, 24.3-51.4) had higher exhaled NO levels than healthy children (15.2±]1.7 ppb; 95% CI, 11.4-18.9). These data indicate that lung oxidative stress is increased in children with asthma treated with inhaled steroids.

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P. Montuschi, L. Ghiro, V. Piovan, S. Carraro, C. Mondino, G. Ciabattoni, E. Baraldi (Rome, Chieti, Italy). Increased exhaled 8-isoprostane in childhood asthma. Eur Respir J 2002; 20: Suppl. 38, 922

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