Source: Annual Congress 2002 - The role of inflammatory markers in children with asthma
Disease area: Airway diseases, Paediatric lung diseases

Abstract

Background: ATS guidelines suggest that mylar balloons are suitable to collect exhaled air for FENO analysis. We studied the effect of humidity and temperature on stability of NO in mylar balloons.
Methods: Exhaled air and dry medical air were stored in 150 ml mylar balloons during 48 hours at 4, 21 and 37 degrees celsius with or without silica gel. Three volunteers with FENO of 12.4 ppb, 21.0 ppb and 34.0 ppb filled 30 balloons each. In addition, balloons were filled with dry NO-free medical air and NO calibration gas of 115 ppb. NO was measured after 0, 6, 9, 24 en 48 h with a chemiluminescence NO-analyser (Sievers 280B). Paired t-tests were used to compare NO values.
Results: FENO remained stable in balloons for 9 hours at all temperatures when no silica gel was added and irrespective of initial values. NO values increased significantly between 9 and 48 h, but only with low initial FENO. Under all storage conditions, silica gel increased variability, even after 6 h. Stability was worst at 37 degrees celsius, in the presence of silica gel. Conclusions FENO in mylar balloons is stable for at least 9 hours, and storage temperature is not critical. With longer storage time, balloons seem to generate NO, dependent on initial FENO. There is no advantage in adding silica gel as it increases variability.
Supported by a grant of the Italian NO Club, Valeas S.p.a. and the Dutch Asthma Foundation.

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M. W. H. Pijnenburg, A. Bodini, A. L. Boner, J. C. De Jongste (Rotterdam, The Netherlands; Verona, Italy). Stability of NO in mylar bags: effect of storage conditions. Eur Respir J 2002; 20: Suppl. 38, 921

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