Source: International Congress 2014 – New mechanisms in the pathogenesis of asthma and other lung diseases
Disease area: Airway diseases

Abstract

Pulmonary Sarcoidosis is characterised by hystiopatological features of noncaseating epithelioid cell granulomas originated from alveolar macrophages. Despite sarcoidosis occurs more frequent in never-smoker subjects than smokers, it has been speculated that unknown inhaled antigens may induce aberrant leukocytes stimulation leading to formation of sarcoid granuloma.Our group of research applied the proteomic approach to the analysis of bronchoalveolar lavage (BAL) in order to contribute to the study of the pathogenetic mechanisms of the disease and to differentiate potential disease biomarkers.BALF samples were resolved by 2D-electrophoresis. Protein identification was carried out by mass spectrometry and adequate statistical analysis and bioinformatic tools were also applied. 9 patients with sarcoidosis, 10 never-smoker (NSC) and 8 smoker controls (SC) were enrolled.55 protein spots, corresponding to 38 proteins, were differentially expressed in patients than controls; among the protein up-regulated in sarcoidosis compared with healthy controls there were alpha2-HS-glycoprotein, ApoAI and transthyretin, meanwhile proteins down-regulated in sarcoidosis than controls were actin cytoplasmic 1 and glutathione S-transferase P. Lysozyme C was differently expressed in sarcoidosis cases than SC and complement C3 was up-regulated in patients and SC than NSC. Enrichment analysis showed several molecular pathways involved in the disease pathogenesis that included complement system, oxidative stress, lipid metabolism and immunoregulation.Interesting proteins and molecular pathways, potentially related to sarcoidosis pathogenesis, were highlighted in this study. Their potential role as prognostic bioindicators has to be further investigated.

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E. Bargagli, C. Landi, A. Carleo, G. Cillis, R. M. Refini, D. Bennett, M. G. Perari, L. Bini, P. Rottoli (Siena, Italy). Proteomic investigation of pulmonary sarcoidosis. Eur Respir J 2014; 44: Suppl. 58, 3878

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