Role of IL-33/ST2 axis in systemic inflammation of allergic airway disease
L. Vocca, A. Sala, S. Gangemi, C. Di Sano, L. Riccobono, C. Uasuf, G. Anzalone, A. Bonanno, R. P. Gagliardo, M. Profita (Palermo, Milan, Messina, Italy)
Source: International Congress 2014 – New mechanisms in the pathogenesis of asthma and other lung diseases
Disease area: Airway diseases
Abstract Introduction: T helper 2 (Th2) lymphocytes play an important role in chronic inflammatory process of allergic disease. IL-33 targeting ST2 receptor selectively expressed on Th2 cells surface, regulates the production of cytokines as IL-17A and IL-31.Aim: To evaluate the role of IL-33/ST2 axis in the IL-31 and IL-17 production during systemic inflammation of patients with allergic rhinitis (AR) and concomitant allergic asthma and rhinitis (AAR) compared to healthy control subjects (HC).Methods: 20 HC, 14 AR and 17 AAR subjects were recruited and blood samples collected. IL-31, IL-33, and IL-17A plasma concentrations were measured by commercial ELISA. IL-17A and IL-31 production, and ST2 receptor expression were evaluated by flow-cytometric analysis in PBMC from HC, AR, AAR (n=6 for each group). Finally, the in vitro activity of Beclomethasone Dipropionate (BDP) (10- 8 M to 10- 7 M) was evaluated on IL-17A and IL-31 production, together with ST2 receptor expression in PBMC from AAR (n=6).Results: IL-17A, IL-31 and IL-33 plasma levels were significantly higher in patients with AR and AAR than in HC. IL-31 and IL-17A intracellular levels significantly increased in PBMC from AR and AAR compared to HC while ST2 receptor expression was significantly lower. Finally, IL-17A and IL-31 intracellular levels were decreased by BDP treatment, while ST2 receptor expression increased in cultured PBMCs from AAR.Conclusions: IL-33/ST2 axis activation is a biomarker of Th2/IL-31 and Th17 immune response in the allergic airway disease. Finally, a potential therapeutic approach by BDP might be useful treatment to control Th2/IL-31 and Th17 immune response, in patients with concomitant allergic asthma and rhinitis.
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L. Vocca, A. Sala, S. Gangemi, C. Di Sano, L. Riccobono, C. Uasuf, G. Anzalone, A. Bonanno, R. P. Gagliardo, M. Profita (Palermo, Milan, Messina, Italy). Role of IL-33/ST2 axis in systemic inflammation of allergic airway disease. Eur Respir J 2014; 44: Suppl. 58, 3876
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