Source: International Congress 2014 – Cell biology 2014
Disease area: Airway diseases

Abstract

Rationale: We have previously shown that neutrophils from Chronic Obstructive Pulmonary Disease (COPD) patients migrate with reduced accuracy (Sapey et al. AJRCCM 2011; 183:1176-86). There is some evidence to suggest chemokine receptors may be differentially in COPD neutrophils. We sought to determine if differences in receptor function may explain the aberrant neutrophilic migration in COPD.Methods: Surface expression of CXCR1, CXCR2 and the fMLP receptor (FPR1) was semi quantified by Fluorescence Activated Cell Sorting (FACS) before and at points throughout a 2 hour stimulation time-course with IL-8 or fMLP. CXCR1 and CXCR2 shedding was quantified by ELISA and localisation was assessed by fluorescence microscopy on adhered and fixed neutrophils following stimulation with fMLP.Results: CXCR1 was significantly lower in quiescent COPD neutrophils compared to health. This difference was rapidly abolished following stimulation with IL-8. No differences were observed in CXCR2 or FPR1 expression. CXCR1 shedding was negligible but there was more CXCR2 shedding detected, although there was no difference between COPD and health. Both COPD and healthy neutrophils appeared to mobilise CXCR1 and CXCR2 to the leading edge once they had been stimulated.



Conclusions: Our novel data on receptor function in stimulated neutrophils suggest that chemokine receptors are unlikely to be an influential factor in the aberrant migration of COPD neutrophils.

Rating:

Share or cite this content

Citations should be made in the following way:
J. Stockley, L. Peat, G. Walton, J. Lord, E. Sapey (Birmingham, United Kingdom). The role of chemokine receptors in the aberrant migration of COPD neutrophils. Eur Respir J 2014; 44: Suppl. 58, 3859

Member's Comments

No comment yet.

Related content which might interest you: Imbalance of immune response functional phenotype and alveolar macrophages phenotype in COPD Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 Lymphocyte senescence in COPD is associated with loss of glucocorticoid receptor expression by cytotoxic/ pro-inflammatory lymphocytes Source: International Congress 2014 – Effect of cigarette smoke on cell behaviour: cell biology, signalling and senescence Year: 2014 Oxidative stress drives defective efferocytosis in COPD M2-like macrophages Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 Expression of CX3CL1 and CX3CR1 in human asthmatic lung in relation to cell survival Source: International Congress 2014 – New mechanisms in the pathogenesis of asthma and other lung diseases Year: 2014 Evidence for a novel kit adhesion domain mediating human mast cell adhesion to structural airway cells Source: International Congress 2014 – Cell biology 2014 Year: 2014 Elevated GM-CSF in COPD may drive defective macrophage phenotype Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 Smoking alters alpha1-antitrypsin in alveolar macrophages possibly promoting misfolding Source: International Congress 2014 – New studies into pulmonary proteases and antiproteases Year: 2014 Comparison of inflammatory markers in induced and spontaneous sputum in COPD patients Source: International Congress 2014 – Translational studies in COPD Year: 2014 COPD airway epithelium secretion attracts and activates neutrophils Source: International Congress 2014 – Novel evidence relating to respiratory treatment development Year: 2014 The effect of budesonide on TLR expression in alveolar macrophages from smokers Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 Interleukin-18 levels in induced sputum of stable and exacerbated COPD patients Source: International Congress 2014 – Translational studies in COPD Year: 2014 Macrophage migration inhibitory factor (MIF), a biomarker in COPD? Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 The role of circulating mitochondrial DNA in modulating airway inflammation of chronic obstructive pulmonary disease Source: International Congress 2014 – Cell biology 2014 Year: 2014 Hyperoxia impairs the inflammatory response of alveolar macrophages to multiple Toll-like receptor ligands associated with bacterial infection Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 Crosstalk between epithelium and fibroblasts; implications for COPD Source: International Congress 2014 – Mechanistic studies of the airway epithelium Year: 2014 CEACAM6-high airway neutrophils and epithelial cells are a feature of severe asthma Source: International Congress 2015 – Translational studies in airway cell biology Year: 2015 Increased expression of receptor for advanced glycation end products in COPD Source: International Congress 2014 – Translational studies in COPD Year: 2014 Differential sensitivity of subsets of monocyte-derived macrophages to apoptosis; its impact on lung inflammation Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 Functional phenotype of immune response and alveolar macrophages phenotype in pulmonary sarcoidosis Source: International Congress 2014 – New insights into monocyte and macrophage biology Year: 2014 LATE-BREAKING ABSTRACT: BAFF: a self-perpetuating mechanism of B cell activation in COPD Source: International Congress 2014 – COPD physiopathology Year: 2014